Original title: Separation and Detection of Bioconjugated Quantum Dots Using on a Chip Electrophoresis
Authors: Pejović, Jelena
Document type: Papers
Language: eng
Publisher: Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
Abstract: Semiconductor nanocrystals, quantum dots (QDs), are nanoscale particles that have been attract a lot of attention due to their unique optic and electronic properties. Due to very diverse and numerous applications, it is really important to make a tool for precise and controlled detection of QDs. In this study, we investigate on a chip detection and separation of QDs bioconjugated with BSA (bovine serum albumin) using homemade equipment based on principle of capillary electrophoresis (CE) and optical detection. Quenching effect of different concentration of BSA on fluorescence intensity of QDs was monitored. It was found that with increasing concentration of BSA fluorescence intensity of QDs is decreasing. This research can lead to a better understanding of interaction between different size QDs and biomolecules.
Keywords: bioconjugation; bovine serum albumin; capillary electrophoresis; Quantum dots
Host item entry: Proceedings of the 22nd Conference STUDENT EEICT 2016, ISBN 978-80-214-5350-0

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/84015

Permalink: http://www.nusl.cz/ntk/nusl-383733


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