Original title: Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation
Authors: GRAHAMMER, Johannes
Document type: Bachelor's theses
Year: 2018
Language: eng
Abstract: The quality of biological samples for Transmission Electron Microscopy prepared using High Pressure Freezing and Freeze Substitution with a novel agitation module invented by Helmuth Goldhammer and Siegfried Reipert was compared to Cells prepared without agitation and the Freeze Substitution protocol by Obornik et al. The amount of extraction was quantified via immunolabelling of RuBisCO inside the plastids of the Alveolate Chromera Velia Mechanical Damages were quantified by counting of obviously damaged and intact cells at low magnification
Keywords: Agitation; Freeze Substitution; High Pressure Freezing; Immunolabeling; RuBisCO
Citation: GRAHAMMER, Johannes. Preparation of Specimen for Transmission Electron Microscopy using Freeze Substitution and Agitation. České Budějovice, 2018. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/49787

Permalink: http://www.nusl.cz/ntk/nusl-381868


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses