Original title: Genetic engineering of \kur{psbA} gene in \kur{Nicotiana tabacum}
Authors: HUCKOVÁ, Dagmar
Document type: Master’s theses
Year: 2017
Language: eng
Abstract: Transformation vector with mutated psbA gene and selective aadA gene was created and transferred into Nicotiana tabacum living cells using Biolistic bombardment. Due to homologous recombination, transformed plant lineage carrying D1-A209, D1-C-212 instead of D1-S209, D1-S212 in D1 protein in PS II was obtained. Seeds from transformed plant were harvested and homoplasmy of the first generation was tested. These mutations caused higher thermostability in Synechocystis sp. PCC6803 so the transformed plant is expected to be the first step in the study of PS II thermostability in higher plants.
Keywords: Biolistics; genetic transformation; psbA gene
Citation: HUCKOVÁ, Dagmar. Genetic engineering of \kur{psbA} gene in \kur{Nicotiana tabacum}. České Budějovice, 2017. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/48786

Permalink: http://www.nusl.cz/ntk/nusl-381806


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses