Original title:
Evaluation of negative stains for single particle analysis in electron microscopy
Authors:
ĎURINOVÁ, Eva Document type: Master’s theses
Year:
2018
Language:
eng Abstract:
Four negative stains, hafnium chloride and europium, samarium and gadolinium nitrates, were tested in single particle electron microscopy as potential alternatives to uranyl acetate, which is recently being widely restricted for its toxicity. The new stains were applied to a structurally well-described plant photosystem I, visualized by a transmission electron microscope and classified in a single particle analysis. The quality of the stains was evaluated by the obtained resolution and ability to provide reliable structural information.
Keywords:
electron microscopy; lanthanides; negative staining; single particle analysis; Uranyl acetate Citation: ĎURINOVÁ, Eva. Evaluation of negative stains for single particle analysis in electron microscopy. České Budějovice, 2018. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/49572