Original title: Comparison of TEM sample preparation methods for immunogold labeling
Authors: GRECHHAMER, Christian
Document type: Bachelor's theses
Year: 2017
Language: eng
Abstract: The tracking of antigens in biological samples can be achieved by labeling them with gold-gonjugated antibodies (immunogold labeling). This thesis gives an overview of several sample preparation methods for TEM and investigates their effects on the binding efficiency of antibodies to certain antigens. For this reason, protein samples were processed according to four different protocols, cut into ultra-thin sections and labeled with primary and secondary antibodies. The micrographs, taken during observation with a TEM, were analysed quantitatively and the obtained data was statistically evaluated.
Keywords: gold quantitation; high pressure freezing; immunogold labeling; labeling density; microtomy; microwave-assisted sample preparation; super quick freeze substitution; Transmission Electron Microscopy
Citation: GRECHHAMER, Christian. Comparison of TEM sample preparation methods for immunogold labeling. České Budějovice, 2017. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/41340

Permalink: http://www.nusl.cz/ntk/nusl-354727


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses