Detekce, rozšíření, diverzita a fylogeneze původce račího moru Aphanomyces astaci (Oomycetes)

Kozubíková, Eva

My Ph.D. thesis deals with various aspects of research of the crayfish plague pathogen, Aphanomyces astaci (Oomycetes). Crayfish plague decimates whole populations of European crayfish; therefore, it is one of the main problems for conservation of these species endangered from other reasons as well. The infection is transmitted by invasive North American crayfish, which are much less susceptible to the disease and are apparently original hosts of A. astaci. The core of this thesis consists of six studies. Four of them have been already published, the remaining two are manuscripts under review. The main linking motive among the presented studies is not only the crayfish plague pathogen itself, but also the use, development and verification of the reliability of molecular detection methods of A. astaci and its genotypes. This core of the thesis is further divided into two parts. The first one contains four studies that deal with the distribution of A. astaci in North American crayfish populations in the Czech Republic (Chapter 1) and Hungary (Chapter 2), and factors influencing this distribution. Two North American crayfish species can be found in the wild in these countries, the spiny-cheek crayfish (Orconectes limosus) and the signal crayfish (Pacifastacus leniusculus). The first two chapters show...

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