Original title: Charakteristika programované buněčné smrti při vzniku lyzigenního aerenchymu
Translated title: Characterization of programmed cell death during lyzigenous aerenchyma formation
Authors: Lenochová, Zuzana ; Votrubová, Olga (advisor) ; Čiamporová, Milada (referee) ; Kummerová, Marie (referee)
Document type: Doctoral theses
Year: 2012
Language: cze
Abstract: Abstract____________________________________________________________________ 2 ABSTRACT: Zea mays is generally considered to be a plant with inducible lysigenous aerenchyma formation. The degradation of some cortical cells is triggered by environmental conditions, usually in the form of stress (submergence etc.). These cells die in a process that shows signs characteristic for programmed cell death, such as nuclear DNA fragmentation or apoptotic ultrastructural alterations. Aerenchyma formed in primary roots of thirteen examined maize accessions, irrespective of cultivation conditions. The aerenchyma fraction correlated with the root length, not with its age. The dependence of aerenchyma formation on the presence of this phytohormone was proved by using an inhibitor of ethylene synthesis (AOA). It was found out that the aerenchyma formation depended on light conditions and that the lysigenous intercellulars intercellular spaces colocalized with areas with cells with characteristically fragmented nuclear DNA (TUNEL-positive nuclei). In experiments using the TUNEL reaction it was necessary to determine new dilution of the enzymatic mixture for the examined plant material. Only the observation of surface planes of free-hand root sections was considered relevant in both TUNEL-TMR and TUNEL-AP assays. TUNEL-AP...
Keywords: aerenchyma; maize; PCD; roots; wetland plants; aerenchym; kořeny; kukuřice; mokřadní rostlny; PCD

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/45189

Permalink: http://www.nusl.cz/ntk/nusl-309479


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses