Original title: Změny onkogenních vlastností nádorových buněk modulací exprese onkogenů. Příklad kuřecích buněčných linií transformovaných genem v-src
Translated title: Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines
Authors: Kovářová, Denisa ; Hejnar, Jiří (advisor) ; Španielová, Hana (referee)
Document type: Master’s theses
Year: 2009
Language: cze
Abstract: 4 Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines Abstract v-Src protein tyrosine kinase is the product of the transforming gene transduced by avian Rous sarcoma virus. In contrast to proto-oncogene c-src, v-src lacks the negative-regulatory C-terminal domain and consequently shows a higher level of activity and transforming ability. In addition, v-Src contains point mutations throughout its coding region that probably contribute to the high level of intrinsic kinase activity. Long terminal repeats (LTR) comprise strong promoter-enhancer sequences and ensure efficient expression of the v-src gene. v-Src protein has a strong transforming potential in vitro and induces tumor development and growth in vivo. Moreover, it is implicated in metastatic formation. In several cancer types the elevated c-Src kinase activity caused pleiotropic cellular responses inducing transformation and metastasis. The aim of this diploma work was to reveal the role of v-Src in mediating tumor and metastatic progression in chicken cell lines PR9692 and PR9692-E9. Despite the low propensity of the chicken cells to immortalization, comparatively high immoratlization efficiency was observed in cells from ex vivo tumours growing progressively...

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/26122

Permalink: http://www.nusl.cz/ntk/nusl-295380


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses