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Original title:
Využití elektroforetických metod v analýze farmaceuticky významných látek
Translated title: Use of electrophoretic methods in the analysis of pharmaceutically important compounds
Authors: Šafra, Jiří ; Pospíšilová, Marie (advisor) ; Jokl, Vladimír (referee) ; Ušelová, Kateřina (referee)
Document type: Doctoral theses
Year: 2008
Language: cze
Abstract: The thesis deals with the use of electrophoretic methods in the analysis of pharmaceutically important compounds. In the theoretical part, the principles of basic techniques (like CZE, MEKC, ITP etc.) as well as on-line preconcentration methods (stacking, sweeping, ITP-CZE, tITP) are described. The first part of the experimental work deals with the use of on-line sample preconcentration techniques (ITP-CZE and LVSS with polarity switching) for the analysis of the complex matrices of plant origin. 1. The on-line ITP-CZE was used for the separation and quantification of phenolic acids derived from benzoic and cinnamic acids in methanolic extract of Epilobium parviflorum. BGE-S-BGE electrolyte system was used for the separation. The leading electrolyte in the ITP pre-separation step was 0.01M HCl, 0.02M imidazol, 0.2% HEC with pH 7.2; the terminating electrolyte was 0.01M HEPES with pH 8.2. The background electrolyte in the electrophoretic step contained 25mM MES, 50mM TRIS, 30mM boric acid, 10mM α-cyclodextrine, 0.2% HEC of pH 8.3. A single analysis took 25 min. No special sample pretreatment was required. 2. The next work focused on the application of a stacking-CZE method used for the separation and determination of eight phenolic acids in an extract of Epilobium parviflorum. Large-volume sample stacking...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/14213
Permalink: http://www.nusl.cz/ntk/nusl-290239
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses
Translated title: Use of electrophoretic methods in the analysis of pharmaceutically important compounds
Authors: Šafra, Jiří ; Pospíšilová, Marie (advisor) ; Jokl, Vladimír (referee) ; Ušelová, Kateřina (referee)
Document type: Doctoral theses
Year: 2008
Language: cze
Abstract: The thesis deals with the use of electrophoretic methods in the analysis of pharmaceutically important compounds. In the theoretical part, the principles of basic techniques (like CZE, MEKC, ITP etc.) as well as on-line preconcentration methods (stacking, sweeping, ITP-CZE, tITP) are described. The first part of the experimental work deals with the use of on-line sample preconcentration techniques (ITP-CZE and LVSS with polarity switching) for the analysis of the complex matrices of plant origin. 1. The on-line ITP-CZE was used for the separation and quantification of phenolic acids derived from benzoic and cinnamic acids in methanolic extract of Epilobium parviflorum. BGE-S-BGE electrolyte system was used for the separation. The leading electrolyte in the ITP pre-separation step was 0.01M HCl, 0.02M imidazol, 0.2% HEC with pH 7.2; the terminating electrolyte was 0.01M HEPES with pH 8.2. The background electrolyte in the electrophoretic step contained 25mM MES, 50mM TRIS, 30mM boric acid, 10mM α-cyclodextrine, 0.2% HEC of pH 8.3. A single analysis took 25 min. No special sample pretreatment was required. 2. The next work focused on the application of a stacking-CZE method used for the separation and determination of eight phenolic acids in an extract of Epilobium parviflorum. Large-volume sample stacking...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/14213
Permalink: http://www.nusl.cz/ntk/nusl-290239
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses
Record created 2017-04-25, last modified 2022-03-04