Hyaluronan v chondrogenezi mezenchymálních kmenových buněk

Dvořáková, Jana

6 SUMMARY OF THE THESIS The main aim of the project was to develop a methodological approach of in-vitro chondrogenic graft preparation. This graft should utilise mesenchymal stem cells (MSC) seeded in a hyaluronan based scaffold. A review of known hyaluronan derivatives with a potential for biological applications was prepared in the first phase of the project. (Sedova, Knotkova et al. 2007) As a result of the review, a hydrogel based on tyramine derivative of hyaluronan cross-linked by peroxidase (HA-TA scaffold) was chosen for the following research. A sufficiently rich, reliable and ethically acceptable source of MSCs was required. Washed bone marrow collection sets remaining after bone marrow collection for transplantation purposes was demonstrated to meet these needs. (Dvorakova, Hruba et al. 2008) MSCs reveal specific features, namely low seeding density and morphological changes during culture, which may affect the outcome of cell viability measurements in experiments. In order to solve this complication, three different methods of cell viability determination were compared, and the luminiscent detection of intracellular ATP was recommended for MSC applications. (Vistejnova, Dvorakova et al. 2009) Hyaluronan influence on chondrogenic differentiation of MSCs was evaluated in a pellet micromass system...

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