Original title: Vliv flubendazolu na aktivitu vybraných biotransformačních enzymů u parazitujících červů Haemonchus contortus
Translated title: Effect of flubendazole on activities of selected biotransformation enzymes in helminth parasite Haemonchus contortus
Authors: Babíková, Martina ; Lamka, Jiří (referee) ; Skálová, Lenka (advisor)
Document type: Master’s theses
Year: 2009
Language: cze
Abstract: [cze] [eng]
Táto diplomová práca sa zaoberá úlohou biotransformačných enzýmov v navodení rezistencie parazitujúcich červov na benzimazolové anthelmintika. Liečba infekcií spôsobených helmintmi sa stala problematickou kvôli častej liekovej rezistencií parazitických červov. Rozvoj rezistencie môže byť podporený činnosťou enzýmov metabolizujúcich xenobiotikum . Ako model bol vybraný Haemonchus contortus, ktorý je jedným z najpatogénnejších parazitov domácich a voľne žijúcich prežúvavcov. Dospelci H. contortus boli izolovaní z nakazených oviec, ktoré boli liečené rôznymi sub-terapeutickými dávkami anthelmintika flubendazolu, alebo z oviec neliečených. V subcelulárnych frakciach homogenátu červov boli experimentálne zistené špecifické aktivity vybraných biotransformačných enzýmov prvej a druhej fázy. Porovnaním hodnôt enzymatickej aktivity u rôznych skupín červov som sa snažila zistiť jej možné ovplyvnenie predošlým kontaktom s flubendazolom This diploma thesis treats of biotransformation enzymes issue in development of resistance of parasitic helminths on benzimidasole anthelmintics. The treatment of helminthic infections has become problematic because of frequent drug resistance of helminth parasites. The development of drug resistance can be facilitated by the action of xenobiotic metabolizing enzymes. Experimental model was represented by Haemochus contortus which is one of the most pathogenic parasites of domestic and wild ruminant species. Adults of H. contortus were isolated from infected sheep; these were treated by sub-therapeutic doses of anthelmintic flubendazole. And were also isolated from infected sheep but with no treatment applied. There were determined specific activities of selected biotransformation enzymes of the first and second phase in subcellular fractions of the hetminth homogenate. Comparing enzymatic activity values among examined groups of hetminths I was evaluating possible influence on enzymatic activities if there was flubendazole applied in the past.

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/27708

Permalink: http://www.nusl.cz/ntk/nusl-282902


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses