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Original title:
Vliv environmentálních stresů na mutabilitu u Bacillus subtilis - role mismatch-repair systému
Translated title: Effect of environmental stresses on mutability of Bacillus subtilis - role of mismatch-repair system
Authors: Nunvář, Jaroslav ; Lichá, Irena (advisor) ; Konopásek, Ivo (referee)
Document type: Master’s theses
Year: 2009
Language: cze
Abstract: The everchanging nature of bacterial environment requires adaptation to emerging novel conditions. One proposed way of adaptation involves increased generation of genetic variability in response to harmful conditions - a phenomenon called adaptive mutagenesis. However, the details of mechanisms of adaptive mutagenesis, and even its very existence, are far from clear. Our goal was to subject the Gram-positive model bacterium Bacillus subtilis to variety of environmental stresses, examine the rate of mutagenesis occuring and compare it to unstressed conditions. Next we wondered if there was a role for mismatch-repair system (MMR), the major pathway for mutation avoidance, in these processes. To accomplish this, we constructed systems to monitor the expression of MMR components both on transcription and translation level. We also developed a mathematical model for precise mutation rate determination in order to quantify the intensity of mutagenic processes. The monitoring of MMR proteins translation failed due to high background endogenous fluorescence present in B. subtilis cells. However, we found out, using transcription reporter system, that the expression of MMR is not influenced by imposition of harsh hyperosmotic shock upon cells. The expression of MMR was also barely influenced by nutrient limitation...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/26183
Permalink: http://www.nusl.cz/ntk/nusl-281272
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Translated title: Effect of environmental stresses on mutability of Bacillus subtilis - role of mismatch-repair system
Authors: Nunvář, Jaroslav ; Lichá, Irena (advisor) ; Konopásek, Ivo (referee)
Document type: Master’s theses
Year: 2009
Language: cze
Abstract: The everchanging nature of bacterial environment requires adaptation to emerging novel conditions. One proposed way of adaptation involves increased generation of genetic variability in response to harmful conditions - a phenomenon called adaptive mutagenesis. However, the details of mechanisms of adaptive mutagenesis, and even its very existence, are far from clear. Our goal was to subject the Gram-positive model bacterium Bacillus subtilis to variety of environmental stresses, examine the rate of mutagenesis occuring and compare it to unstressed conditions. Next we wondered if there was a role for mismatch-repair system (MMR), the major pathway for mutation avoidance, in these processes. To accomplish this, we constructed systems to monitor the expression of MMR components both on transcription and translation level. We also developed a mathematical model for precise mutation rate determination in order to quantify the intensity of mutagenic processes. The monitoring of MMR proteins translation failed due to high background endogenous fluorescence present in B. subtilis cells. However, we found out, using transcription reporter system, that the expression of MMR is not influenced by imposition of harsh hyperosmotic shock upon cells. The expression of MMR was also barely influenced by nutrient limitation...
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/26183
Permalink: http://www.nusl.cz/ntk/nusl-281272
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Record created 2017-04-25, last modified 2022-03-03