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Original title:
Model fokální ischemické léze mozku u neonatálních potkanů
Translated title: Model of focal ischemic brain lesion in neonatal rat.
Authors: Hráchová, Anna ; Brime, Tufikameni (referee) ; Otáhal, Jakub (advisor)
Document type: Master’s theses
Year: 2010
Language: eng
Abstract: Title of work: Model of focal brain ischemic lesion in neonatal rats. Work objectives: The aim of this diploma thesis was to determinate the parameter of the lesion after focal brain ischemia induced by photothrombosis, establish the changes of sensorimotor abilities and investigate the occurrence of post stroke epileptic seizures in this new model in P7 rat pups. Materials and Methods: Intravenous application of bengal rose dye was followed by illumination of concentrated beam of high powered green laser over the sensorimotocortex for 5 minutes at 0,5s on/off cycles. To assess the motor deficit, rotarod walking test and bar holding test were performed in two months after stroke. The animals underwent implantation of EEG cortical and hippocampal electrodes followed by a week of recovery four months after stroke. EEG monitoring for five consecutive days have been done. Then animals were overdosed with urethane and transcardially perfused. The brains were cut into 50μm slice for histological staining. There were another five previous experiments, which were preliminary studies aiming at correct inducing of photothrombotic stroke and also EEG monitoring at all. Every study included a control groups that underwent the same surgical protocol. Present work includes only the last sixth experiment....
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/22208
Permalink: http://www.nusl.cz/ntk/nusl-276918
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Translated title: Model of focal ischemic brain lesion in neonatal rat.
Authors: Hráchová, Anna ; Brime, Tufikameni (referee) ; Otáhal, Jakub (advisor)
Document type: Master’s theses
Year: 2010
Language: eng
Abstract: Title of work: Model of focal brain ischemic lesion in neonatal rats. Work objectives: The aim of this diploma thesis was to determinate the parameter of the lesion after focal brain ischemia induced by photothrombosis, establish the changes of sensorimotor abilities and investigate the occurrence of post stroke epileptic seizures in this new model in P7 rat pups. Materials and Methods: Intravenous application of bengal rose dye was followed by illumination of concentrated beam of high powered green laser over the sensorimotocortex for 5 minutes at 0,5s on/off cycles. To assess the motor deficit, rotarod walking test and bar holding test were performed in two months after stroke. The animals underwent implantation of EEG cortical and hippocampal electrodes followed by a week of recovery four months after stroke. EEG monitoring for five consecutive days have been done. Then animals were overdosed with urethane and transcardially perfused. The brains were cut into 50μm slice for histological staining. There were another five previous experiments, which were preliminary studies aiming at correct inducing of photothrombotic stroke and also EEG monitoring at all. Every study included a control groups that underwent the same surgical protocol. Present work includes only the last sixth experiment....
Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/22208
Permalink: http://www.nusl.cz/ntk/nusl-276918
The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
Record created 2017-04-25, last modified 2022-03-03