Original title: Výskyt RNA elementů u rodu Armillaria
Translated title: RNA elements in the genus Armillaria
Authors: Dvořák, Jiří ; Gabriel, Jiří (advisor) ; Tomšovský, Michal (referee)
Document type: Master’s theses
Year: 2008
Language: cze
Abstract: Mycoviruses are widespread among the fungi. Most mycoviruses reported have dsRNA genomes. The first mycovirus was found in the edible mushroom Agaricus bisporus. Mycoviruses do not have an extracellular phase of their life cycle and are transmitted only by intracellular routes. Many mycoviruses reveal no apparent effect on their hosts. The genus Armillaria represents economically important edible fungal pathogens causing root rot in conifers and broadleaves trees. From this viewpoint, genus Armillaria presents an important subject of research studies. In this study we tried to find evidence for the presence of RNA elements in the respective genus. Total amount of collected strains was 52. CF-11 method for nuclei acids extraction was applied to 40 strains. The method is based on the affinity of cellulose powder for nuclei acids and specifically, the adsorption of dsRNA at ethanol concentrations of 15%. According to our results, there is no evidence for dsRNA elements occurence in the tested strains. The study was also focused on basic characterization of physiology of isolates, esp. to optimum growth conditions on agar plates and milled straw as well as on rhizomorph formation and fructification. No effect of pH changes and addition of various alcohols on rhizomorph formation was found, while primordia...

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/1281

Permalink: http://www.nusl.cz/ntk/nusl-272554


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses