Original title: Zavedení a optimalizace \kur{in vivo} modelů zánětu a jejich využití pro funkční analýzu inhibitorů proteáz z klíštěcích slin
Authors: CHLASTÁKOVÁ, Adéla
Document type: Master’s theses
Year: 2016
Language: cze
Abstract: Two murine models of acute inflammation, namely thioglycollate-induced peritonitis and carrageenan-induced paw edema, were optimized using non-steroidal anti-inflammatory drug indomethacin and corticosteroid dexamethasone. During the optimization phase, the presence of neutrophils, monocytes, macrophages, eosinophils, B cells and T cells in the peritoneal cavity at various time points after injection of thioglycollate medium was assessed via multicolor flow cytometry. Moreover, two different thioglycollate media (suppliers BD and Sigma-Aldrich) were compared for their ability to induce an inflammatory response. The optimization of thioglycollate-induced peritonitis and carrageenan-induced paw edema was followed by the evaluation of the anti-inflammatory activity of Ixodes ricinus cystatins G1 and G9 in both mouse models.
Keywords: carrageenan-induced paw edema; cystatins; eosinophils; Ixodes ricinus; monocytes; neutrophils; thioglycollate-induced peritonitis
Citation: CHLASTÁKOVÁ, Adéla. Zavedení a optimalizace \kur{in vivo} modelů zánětu a jejich využití pro funkční analýzu inhibitorů proteáz z klíštěcích slin. České Budějovice, 2016. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/41585

Permalink: http://www.nusl.cz/ntk/nusl-262676


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses