Original title:
Study of 2-Nitrofluorene Interaction with DNA at a Glassy Carbon Electrode
Authors:
Skalová, Štěpánka ; Stávková, K. ; Vyskočil, V. ; Barek, J. Document type: Papers Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year:
2016
Language:
eng Abstract:
2-Nitrofluorene (2-NF) is a nitrated polycyclic aromatic hydrocarbon (NPAH) which occurs as the environmental pollutant. It is a potential carcinogen and mutagen. Interaction of deoxyribonucleic acid (DNA) with 2-NF was monitored using an electrochemical DNA biosensor prepared from a glassy carbon electrode (GCE) and low-molecular-weight DNA by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and square-wave voltammetry (SWV).\nThere were no damaging interactions observed between DNA and 2-NF using EIS. However, CV shows intercalation of this substance into the DNA structure to form the complex 2-NF–DNA. Intercalation was also observed by SWV, confirming intercalation to reduce the number of electroactive sites and thus reducing the peak heights of adenosine and guanosine.
Keywords:
DNA biosensor; glassy carbon electrode; voltammetry Project no.: GBP206/12/G151 (CEP) Funding provider: GA ČR Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5
Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0259939