Original title: Searching for a nuclear transportin of the chromatin-remodeling factor ISW1 in Saccharomyces cerevisiae
Translated title: Vyhledávání jaderného transportinu faktoru ISW1 remodelujícího chromatin u Saccharomyces cerevisiae
Authors: Strádalová, Vendula ; Hašek, Jiří ; Janatová, Ivana
Document type: Papers
Conference/Event: Symposium of the Society for Histochemistry /46./, Prague (CZ), 2004-09-22 / 2004-09-25
Year: 2004
Language: eng
Abstract: [eng] [cze]
Several intragenously-tagged versions of the ISW1 gene were constructed to follow Isw1p localization either in living (GFP-fusions) or in fixed cells (c-Myc-fusions). The whole set of available importin mutants was transformed with vectors carrying an intragenously-tagged ISW1 gene. In the strains where the importins Kap120p, Nmd5p, Sxm1p, Kap114p, Los1p, Msn5p, Kap123p and Pdr6 were deleted, the Isw1p-GFP fusion regulated by the ISW1 promoter was localized always in the nucleus. This suggests that none from these importins is required for nuclear localization of Isw1p. The localization of the Isw1p-GFP in the mtr10-1 strain differed from that observed in all the other analyzed importin mutants. Our results indicate that Mtr10p could be a good candidate for Isw1p importin. Bylo připraveno několik intragenově značených verzí Isw1, jejichž distribuce byla studována na živých či fixovaných buňkách. Kompletní sada importinových mutantů byla těmito konstrukty transformována. V delečních mutantech importinů Kap120p, Nmd5p, Sxm1p, Kap114p, Los1p, Msn5p, Kap123p a Pdr6 jsme zjistili, že Isw1-GFP byl vždy správně lokalizován do buněčného jádra, což ukazuje, že tyto importiny nejsou do transporu zapojeny.
Keywords: isw1p; saccharomyces cerevisiae
Project no.: CEZ:AV0Z5020903 (CEP), IAA5020409 (CEP), IAA5020102 (CEP)
Funding provider: GA AV ČR, GA AV ČR
Host item entry: Symposium of the Society for Histochemistry /46./

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0015256

Permalink: http://www.nusl.cz/ntk/nusl-20337


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Research > Institutes ASCR > Institute of Microbiology
Conference materials > Papers