Original title: Influence of dietary components and redox enzymes on intestinal microbiota proliferation in the tick \kur{Ixodes ricinus}
Translated title: Influence of dietary components and redox enzymes on intestinal microbiota proliferation in the tick \kur{Ixodes ricinus}
Authors: KUČERA, Matěj
Document type: Master’s theses
Year: 2015
Language: cze
Abstract: [cze] [eng]
Tato diplomová práce je zaměřená na posouzení vlivu složek diety na mikrobiální proliferaci ve střevech klíštěte Ixodes ricinus. Byla posouzena dynamika patogenu Borrelie afzelii v závislosti na přítomnosti/nepřítomnosti hemoglobinu v potravě. Byl demonstrován vliv střevního enzymu, duální oxidázy, na regulaci střevní bakteriální mikroflóry. Na základě výsledků RNA interference byl rovněž naznačen vliv duální oxidázy na ovipozici I. ricinus. Dodatek této práce popisuje optimalizaci klíštěcího in vitro krmeni pro nymfy I. ricinus. In this work, we have analysed the temporal dynamics of gut-dwelling bacteria and Borrelia in the gut of the deer tick Ixodes ricinus. Using quantitative PCR, we have shown that levels of the tick intestinal microflora are profoundly decreased at later stages of feeding on whole blood but not on serum. Even though we noted that host complement system manages to interfere with Borrelia viability in vitro, we did not see any effect of host complement on Borrelia acquisition in adult ticks in vivo. However, we revealed that host hemoglobin is essential for Borrelia proliferation in the tick gut. All together, these data imply that, during feeding, levels of gut-dwelling bacteria and Borrelia are determined by the host. While hemoglobin seems to be detrimental for gut-dwelling bacteria, Borrelia require it in order to proliferate. During off-host stage, we showed that levels of gut-dwelling microflora are regulated by an intestinal transmembrane enzyme Dual oxidase. In conclusion, we aimed, and mostly succeeded, to perform pilot experiments describing the biology of a complex process of regulating gut microflora in the vector Ixodes ricinus and extend it by its impact on Borrelia acquisition
Keywords: complement; dual oxidase; DUOX; heme; hemoglobin; in vitro feeding; Ixodes ricinus; NADPH oxidase; DUOX; duální oxidáza; hem; hemoglobin; in vitro krmeni; Ixodes ricinus; komplement; NADPH oxidáza
Citation: KUČERA, Matěj. Influence of dietary components and redox enzymes on intestinal microbiota proliferation in the tick \kur{Ixodes ricinus}. České Budějovice, 2015. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/35031

Permalink: http://www.nusl.cz/ntk/nusl-187597


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses