Original title: Cílená mutageneze endogenního genu v genomu \kur{D. melanogaster} programovatelnými nukleázami
Translated title: Targeted mutagenesis of the endogenous gene in \kur{D. melanogaster} genome by engineered nucleases
Authors: RENNER, Marek
Document type: Master’s theses
Year: 2015
Language: cze
Abstract: Several techniques have recently been described for precise mutagenesis of selected target sites in the genome. This thesis establishes the method of gene targeting by CRISPR/Cas system in D. melanogaster and compares it with gene targeting using TALENs. To test the mutagenesis systems, we choose an endogenous gene encoding concentrative nucleoside transporter gene (CNT1). We have received two mutants containing large deletions affecting the N-terminal part of the CNT1 gene. We show that CRISPR/Cas is useful tool for targeted gene disruption in D. melanogaster.
Keywords: CNT1; CRISPR; Cílená mutageneze; programované nukleázy; TALEN
Citation: RENNER, Marek. Cílená mutageneze endogenního genu v genomu \kur{D. melanogaster} programovatelnými nukleázami. České Budějovice, 2015. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/34509

Permalink: http://www.nusl.cz/ntk/nusl-187590


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses