Original title: In vitro stanovení antimikrobiální, antioxidativní a protizánětlivé aktivity chinonů ze semen černuchy seté
Translated title: In vitro determination of antimicrobial, antioxidative and antiphlogistic aktivity of quinones from seeds of Nigella sativa
Authors: Kokoška, L. ; Maršík, Petr ; Landa, Přemysl ; Tesařová, H. ; Svobodová, B. ; Halamová, K. ; Flesar, J. ; Přibylová, Marie
Document type: Papers
Year: 2010
Language: cze
Abstract: [cze] [eng]
V rámci této studie jsme prokázali antimikrobiální, antioxidativní a protizánětlivé účinky chinonů ze semen čemuchy seté. Na základě dosažených výsledků se domníváme, že THQ a TQ jsou perspektivními látkami pro využití v potravinářském nebo ve farmaceutickém průmyslu. The in vitro anti-yeast, antioxidant, and anti-inf1ammatory activities ofblack cumin (Nigella sativa) seed quinones, namely dithymoquinone (DTQ), thymohydroquinone (THQ), and thymoquinone (TQ), were evaluated in frame of this work. The results showed that THQ and TQ possessed significant anti-yeast activity and affected the growth of aH strains tested at two pH levels with minimum inhibitory concentrations ranging from 8 to 128 !lg/ml. The best scavenging activity was produced by THQ, which showed a remarkable activity of 2.60 Trolox equivalents (TE) in a concentration range between 1.6 and 6.4 !lg/mL and lCso value of 2.4 !lg/mL in ORAC and DPPH assays, respectively. Contrastingly, TQ possessed only weak DPPH scavenging efficacy (ICso = 170 !lg/mL) but significant antioxidative action of 1.91 TE in ORAC assay. In anti-inf1ammatory assay, THQ and TQ possessed significant inhibitory activity against COX-l and -2 at the concentrations comparable with indomethacin. The data presented in this work suggest that THQ and TQ are biologically effective agents with potential for application in food or phalmaceutical industries.
Keywords: chinons; dihydrothymochinon; Nigella sativa
Project no.: GA525/08/1179 (CEP)
Funding provider: GA ČR
Host item entry: XVI. Odborný seminář s mezinárodní účastí aktuální otázky pěstování, zpracování a využití léčivých, aromatických a kořeninových rostlin, ISBN 978-80-213-2121-2

Institution: Institute of Experimental Botany AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0243622

Permalink: http://www.nusl.cz/ntk/nusl-180633


The record appears in these collections:
Research > Institutes ASCR > Institute of Experimental Botany
Conference materials > Papers