Original title:
GenEx Software library for object detection in genetical analysis evaluation: User manual
Authors:
Schier, Jan ; Kovář, Bohumil ; Kuneš, Michal Document type: Research reports
Year:
2014
Language:
eng Series:
Technical Report Abstract:
This technical report contains user manual for the GenEx library for evaluation of FISH microscopy images. The purpose of the library is to find and filter objects in images from fluorescence microscope and to detect fluorescence signals contained in these objects. The library has been designed to be used for diagnostics of the Turner syndrome chromosome aneuploidies in chromosome X, in other words, to find the (roughly round) interphase nuclei and to count the associated hybridization signals. It is characterized by the following features: - separate processing channels for DAPI and for the fluorescence signals. - no restrictions on the number of the signal channels - output in the form of a CSV-file.
Keywords:
Fluorescence microscopy; image processing; ImageJ; Particle analysis; Segmentation Project no.: TA01010931 (CEP) Funding provider: GA TA ČR