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Original title:
Scavenger receptor - trypsinová peptidáza IrSRP-1 z klíštěte \kur{I. ricinus}
Translated title: Scavenger receptor - trypsine-like protease IrSRP1 from the tick \kur{Ixodes ricinus}
Authors: SINGEROVÁ, Barbora
Document type: Master’s theses
Year: 2013
Language: cze
Abstract: Scavenger receptors are a large family of structurally diverse molecules that have been implicated in a range of biological functions. In this work, a newly identified multi-domain scavenger receptor-serine protease IrSRP-1 from the tick Ixodes ricinus is characterized. IrSRP-1 is related to the serine protease Sp22D from the mosquito Anopheles gambiae. IrSRP-1 is expressed mainly in the tick gut but also in hemocytes, Malpighian tubules, tracheas and ovaries of fully fed females. This was confirmed with Western blots and immunohistological labeling with antibodies raised against recombinantly expressed IrSRP-1 trypsine-like domain. According to acquired qRT-PCR profiles relative expression of IrSRP-1 is strongly up-regulated during female feeding and remains unchanged in ticks experimentally injected with various microbes. Functional characterization by RNA interference revealed that lowering IrSRP1 expression leads to a higher mortality rate during tick female feeding.
Keywords: I. ricinus; scavenger receptor; serine protease; I. ricinus; scavenger receptor; serinová proteáza
Citation: SINGEROVÁ, Barbora. Scavenger receptor - trypsinová peptidáza IrSRP-1 z klíštěte \kur{I. ricinus}. České Budějovice, 2013. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/30786
Permalink: http://www.nusl.cz/ntk/nusl-166288
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Translated title: Scavenger receptor - trypsine-like protease IrSRP1 from the tick \kur{Ixodes ricinus}
Authors: SINGEROVÁ, Barbora
Document type: Master’s theses
Year: 2013
Language: cze
Abstract: Scavenger receptors are a large family of structurally diverse molecules that have been implicated in a range of biological functions. In this work, a newly identified multi-domain scavenger receptor-serine protease IrSRP-1 from the tick Ixodes ricinus is characterized. IrSRP-1 is related to the serine protease Sp22D from the mosquito Anopheles gambiae. IrSRP-1 is expressed mainly in the tick gut but also in hemocytes, Malpighian tubules, tracheas and ovaries of fully fed females. This was confirmed with Western blots and immunohistological labeling with antibodies raised against recombinantly expressed IrSRP-1 trypsine-like domain. According to acquired qRT-PCR profiles relative expression of IrSRP-1 is strongly up-regulated during female feeding and remains unchanged in ticks experimentally injected with various microbes. Functional characterization by RNA interference revealed that lowering IrSRP1 expression leads to a higher mortality rate during tick female feeding.
Keywords: I. ricinus; scavenger receptor; serine protease; I. ricinus; scavenger receptor; serinová proteáza
Citation: SINGEROVÁ, Barbora. Scavenger receptor - trypsinová peptidáza IrSRP-1 z klíštěte \kur{I. ricinus}. České Budějovice, 2013. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/30786
Permalink: http://www.nusl.cz/ntk/nusl-166288
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Record created 2013-12-26, last modified 2023-01-15