Original title: Charakterizace a funkce faktoru C z klíštěte \kur{Ixodes ricinus}
Translated title: Characterization and function of Factor C from the tick \kur{Ixodes ricinus}
Authors: HARTMANN, David
Document type: Master’s theses
Year: 2013
Language: cze
Abstract: Factor C is a multi-domain serine protease which recognizes Gram-negative bacteria via binding to lipopolysaccharides and triggers hemolymph clotting cascade in the horseshoe crab. A closely related molecule was also found to be present in the genome of the tick Ixodes scapularis. In this work, the full sequence of Factor C ortholog from Ixodes ricinus (IrFC) was determined. IrFC is mainly expressed in tick hemocytes and the heavy chain of the activated molecules is present in tick hemolymph as confirmed by Western blotting with antibodies raised against recombinant fragments of IrFC. The function of the IrFC in tick innate immunity was assessed using its silencing by RNA interference.
Keywords: Factor C; hemolymph clotting; Ixodes ricinus; tick; Faktor C; ixodes ricinus; klíště; koagulace hemolymfy
Citation: HARTMANN, David. Charakterizace a funkce faktoru C z klíštěte \kur{Ixodes ricinus}. České Budějovice, 2013. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/30500

Permalink: http://www.nusl.cz/ntk/nusl-166284


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses