Original title: Molecular and biochemical characterization of serine protease SmSP1 in \kur{Schistosoma mansoni}
Authors: OPAVSKÝ, David
Document type: Master’s theses
Year: 2013
Language: eng
Abstract: SmSP1 is a chimerical serine protease consisted of three domains (cub, LDLa and trypsin-like) and found in Schistosoma mansoni. Its characterization was performed by molecular techniques such as PCR screen, qRT-PCR and RNA interference (RNAi) to gain information about expression profile, level expression and susceptibility to RNAi. Further, protein expression was carried out to gain an antigen for immunization and recombinant for biochemical studies. Results of PCR screen and qRT-PCR suggested possible function of SmSP1 in egg and adult stages but SmSP1 gene was not found susceptible to RNAi in NTS. Recombinant from E. coli was successfully used for immunization. Active recombinant was likely expressed in Pichia pastoris but expression conditions are unstable and expression optimization is necessary.
Keywords: antigen; immunization; PCR screen; qRT-PCR; RNA interference; Schistosoma mansoni; serine protease
Citation: OPAVSKÝ, David. Molecular and biochemical characterization of serine protease SmSP1 in \kur{Schistosoma mansoni}. České Budějovice, 2013. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/18616

Permalink: http://www.nusl.cz/ntk/nusl-152946


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses