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Interaction of trimethylchitosan with Niaproof surfactant
Zbořilová, Hana ; Krouská, Jitka (referee) ; Mravec, Filip (advisor)
This thesis is focused on the study of interaction of the polycation N,N,N-trimethylchitosan (TMC) with the anionic surfactant Niaproof® 4 in water and physiological saline solution. Due to the commercial unavailability of N,N,N-trimethylchitosan, the polymer was first synthesized from chitosan, followed by NMR and FTIR characterization. Before the study of the system polycation–anionic surfactant itself, the behavior of the anionic surfactant Niaproof® 4 in aqueous solution and physiological saline solution was explored. The micellization of the surfactant Niaproof® 4 and the aggregation of TMC–Niaproof® 4 system were observed by fluorescence spectroscopy using the fluorescence probe pyrene. Values of critical micelle and critical aggregation concentration were determined on the basis of measurements. It was confirmed that presence of the sodium chloride supports the micellization by increasing the ionic strength of the solution, which leads to decrease of CMC in physiological saline solution. The interaction of TMC–Niaproof® 4 occurs at lower concentration then CMC in both aqueous and physiological environments. Phase separation occurs at higher concentrations of the surfactant leading to stabilization of the system.
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Proposal for Intercompany Communication Change in a Multinational Company Operating in IT Services
Zbořilová, Hana ; Peřinková, Jana (referee) ; Konečná, Zdeňka (advisor)
Diplomová práce se zabývá návrhem změny vnitropodnikové komunikace ve vybrané mezinárodní společnosti působící v oblasti IT služeb. Předmětem výzkumu v analytické části je zmapování vztahů místních zaměstnanců k jejich vedoucím a kolegům pocházejících z odlišných národních kultur. Dále je pak zkoumán vliv kulturních odlišností na aplikovaný způsob řízení a komunikaci v rámci mezinárodního týmu. Na základě výsledků výzkumu provedeného mezi zaměstnanci společnosti formou dotazníkového šetření jsou v návrhové části společnosti doporučena opatření vedoucí k zefektivnění komunikace v rámci společnosti a zefektivnění spolupráce v rámci multikulturního týmu.
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Study of membrane properties of liposomal systems using fluorescence spectroscopy
Zbořilová, Hana ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
The presented diploma thesis is focused on the preparation, characterization and study of membrane properties of liposomal systems which were composed of the neutral phosphatidylcholine (DPPC), cholesterol, negatively charged phosphatidylglycerol (DPPG), polyethylenglycol bounded to phosphatidylethanolamine (PEG5000–PE) and polycation N,N,N-trimethylchitosan (TMC). The influence of individual components and their concentrations on the average particle size, zeta potential and changes in the outer and inner part of the bilayer was investigated. In this matter, methods of dynamic and electrophoretic light scattering and fluorescence spectroscopy with the application of laurdan and DPH probes were used. Based on the above-mentioned parameters, concentrations of components that most suitably influence properties of liposomes in terms of the intended application were selected for the definite complex. It was managed to prepare a liposomal complex stealth liposome–N,N,N-trimethylchitosan, which, due to the optimized composition, could have suitable attributes as a drug delivery system for inhalation administration of biologically active substances.
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Study of membrane properties of liposomal systems using fluorescence spectroscopy
Zbořilová, Hana ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
The presented diploma thesis is focused on the preparation, characterization and study of membrane properties of liposomal systems which were composed of the neutral phosphatidylcholine (DPPC), cholesterol, negatively charged phosphatidylglycerol (DPPG), polyethylenglycol bounded to phosphatidylethanolamine (PEG5000–PE) and polycation N,N,N-trimethylchitosan (TMC). The influence of individual components and their concentrations on the average particle size, zeta potential and changes in the outer and inner part of the bilayer was investigated. In this matter, methods of dynamic and electrophoretic light scattering and fluorescence spectroscopy with the application of laurdan and DPH probes were used. Based on the above-mentioned parameters, concentrations of components that most suitably influence properties of liposomes in terms of the intended application were selected for the definite complex. It was managed to prepare a liposomal complex stealth liposome–N,N,N-trimethylchitosan, which, due to the optimized composition, could have suitable attributes as a drug delivery system for inhalation administration of biologically active substances.
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Interaction of trimethylchitosan with Niaproof surfactant
Zbořilová, Hana ; Krouská, Jitka (referee) ; Mravec, Filip (advisor)
This thesis is focused on the study of interaction of the polycation N,N,N-trimethylchitosan (TMC) with the anionic surfactant Niaproof® 4 in water and physiological saline solution. Due to the commercial unavailability of N,N,N-trimethylchitosan, the polymer was first synthesized from chitosan, followed by NMR and FTIR characterization. Before the study of the system polycation–anionic surfactant itself, the behavior of the anionic surfactant Niaproof® 4 in aqueous solution and physiological saline solution was explored. The micellization of the surfactant Niaproof® 4 and the aggregation of TMC–Niaproof® 4 system were observed by fluorescence spectroscopy using the fluorescence probe pyrene. Values of critical micelle and critical aggregation concentration were determined on the basis of measurements. It was confirmed that presence of the sodium chloride supports the micellization by increasing the ionic strength of the solution, which leads to decrease of CMC in physiological saline solution. The interaction of TMC–Niaproof® 4 occurs at lower concentration then CMC in both aqueous and physiological environments. Phase separation occurs at higher concentrations of the surfactant leading to stabilization of the system.
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Proposal for Intercompany Communication Change in a Multinational Company Operating in IT Services
Zbořilová, Hana ; Peřinková, Jana (referee) ; Konečná, Zdeňka (advisor)
Diplomová práce se zabývá návrhem změny vnitropodnikové komunikace ve vybrané mezinárodní společnosti působící v oblasti IT služeb. Předmětem výzkumu v analytické části je zmapování vztahů místních zaměstnanců k jejich vedoucím a kolegům pocházejících z odlišných národních kultur. Dále je pak zkoumán vliv kulturních odlišností na aplikovaný způsob řízení a komunikaci v rámci mezinárodního týmu. Na základě výsledků výzkumu provedeného mezi zaměstnanci společnosti formou dotazníkového šetření jsou v návrhové části společnosti doporučena opatření vedoucí k zefektivnění komunikace v rámci společnosti a zefektivnění spolupráce v rámci multikulturního týmu.
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Franchising as a method of entering the market
Zbořilová, Hana ; Turnerová, Lenka (advisor) ; Khelerová, Vladimíra (referee)
The bachelor work deals with franchising as a method of entering the market and a certain alternative of small and medium-sized business. It describes the history of franchising, defines notions used in the realm of franchising and analyzes the advantages and disadvatages of this business method. The practical part of the bachelor work deals with describtion of penetrating of franchising in the Czech republic and its developing on the czech market. A czech franchise system from the realm of gastronomy is described in the final part of the work.
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