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Parasitic fungi on selected invasive weeds
Forejtová, Zuzana ; Marková, Jaroslava (advisor) ; Sumíková, Taťána (referee)
Parasitic fungi on selected invasive weeds Zuzana Forejtová Abstract This thesis deals with the invasive plant species problems and their pathogens in the Czech Republic. Special attention is given to weed species colonizing the agroecosystems. The modes and consequences of plant invasions are presented in theoretical part. Practical part includes a list and descriptions of found parasitic fungal pathogens colonizing selected invasive weeds. Moreover, this thesis can be used for educational purposes. In the last part, fungal pathogens of weeds and their use as potential agents in the biological control are discussed. Keywords: invasive plants, invasion, weeds, biological control, fungal pathogens, parasitic fungi
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Distribution and importance of invasive pathogen Cryptostroma corticale on Acer pseudoplatanus in Prague
Kelnarová, Ivana ; Koukol, Ondřej (advisor) ; Sumíková, Taťána (referee)
Sooty bark disease (SBD) caused by pathogen Cryptostroma corticale is one of the current risks to the population of sycamores in Europe. A few dozen individuals were fallen death due to sooty bark disease from the first record in Prague in 2005. However a higher rate of infection in a latent non-symptomatic stage can be assumed. The main goal of the thesis was to quantify the extent of infection C. corticale in Prague on the basis of early detection in tissues of symptomless trees and identify its environmental requirements and stress factors conditioning the progression of the disease. Another goal was to predict the risk for Prague's population of sycamore based on obtained data and identify other vulnerable sites in Prague. A secondary aim was to assess the spectrum of fungi inhabiting xylem of sycamore. Tissue samples collected with an increment borer were subjected to: a) isolation of cultivable fungi on malt agar and b) detection of the DNA C. corticale based on nested PCR with species- specific primers. Latent infection was detected in 28 of the cores. Of 27 positive detections with primer specific nested PCR 10 were supported by isolation of C. corticale on agar plates. Only in one case positive isolation of C. corticale on agar plate was not confirmed by nested PCR. Occurrence of C....
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Distribution and importance of invasive pathogen Cryptostroma corticale on Acer pseudoplatanus in Prague
Kelnarová, Ivana ; Koukol, Ondřej (advisor) ; Sumíková, Taťána (referee)
Sooty bark disease (SBD) caused by pathogen Cryptostroma corticale is one of the current risks to the population of sycamores in Europe. A few dozen individuals were fallen death due to sooty bark disease from the first record in Prague in 2005. However a higher rate of infection in a latent non-symptomatic stage can be assumed. The main goal of the thesis was to quantify the extent of infection C. corticale in Prague on the basis of early detection in tissues of symptomless trees and identify its environmental requirements and stress factors conditioning the progression of the disease. Another goal was to predict the risk for Prague's population of sycamore based on obtained data and identify other vulnerable sites in Prague. A secondary aim was to assess the spectrum of fungi inhabiting xylem of sycamore. Tissue samples collected with an increment borer were subjected to: a) isolation of cultivable fungi on malt agar and b) detection of the DNA C. corticale based on nested PCR with species- specific primers. Latent infection was detected in 28 of the cores. Of 27 positive detections with primer specific nested PCR 10 were supported by isolation of C. corticale on agar plates. Only in one case positive isolation of C. corticale on agar plate was not confirmed by nested PCR. Occurrence of C....
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Methodology of using molecular markers for detection of resistance genes to wheat rusts, powdery mildew and eyspot, and dwarfing genes of wheat
Sumíková, Taťána ; Dumalasová, Veronika ; Trávníčková, Martina
The aim of the publication is to summarize detailed protocols for detection of following resistance genes to wheat rusts, powdery mildew and eyspot (Lr10, Lr19, Lr24, Lr26+Sr31+Yr9+Pm8, Lr28, Lr34+Yr18, Lr37+Yr17+Sr38, Pch1), and dwarfing genes of wheat (Rht-B1 a Rht-D1) using molecular markers based on polymerase chain reaction (PCR). The protocoles are reliable optimized for routine use in a common laboratory with basic equipment needed for PCR.
Fulltext:  PDF
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Parasitic fungi on selected invasive weeds
Forejtová, Zuzana ; Marková, Jaroslava (advisor) ; Sumíková, Taťána (referee)
Parasitic fungi on selected invasive weeds Zuzana Forejtová Abstract This thesis deals with the invasive plant species problems and their pathogens in the Czech Republic. Special attention is given to weed species colonizing the agroecosystems. The modes and consequences of plant invasions are presented in theoretical part. Practical part includes a list and descriptions of found parasitic fungal pathogens colonizing selected invasive weeds. Moreover, this thesis can be used for educational purposes. In the last part, fungal pathogens of weeds and their use as potential agents in the biological control are discussed. Keywords: invasive plants, invasion, weeds, biological control, fungal pathogens, parasitic fungi
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