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Methodology sanitation currants from viruses and phytoplasmas
Sedlák, J. ; Špak, Josef ; Paprštein, F. ; Přibylová, Jaroslava ; Svobodová, L. ; Kubelková, Darina
The aim of this work was to determine the health status of primary resources of 4 red currant cultivars, 1 white currant cultivar and 3 black currant cultivars and research of thermotherapy in 39 °C as a method of sanitation from viral diseases. BRV (Black currant reversion virus) and GVBaV (Gooseberry vein banding associated virus) were detected in some primary resources by PCR. SLRSV (Strawberry latent ringspot virus) was detected by ELISA testing in one bush of a red currant. Phytoplasmas were not present in tested plants. Based on the preliminary results of testing, one clone of red currant cultivar ´Rubigo´ was sanitated from GVBaV, one clone of red currant cultivar ´Detvan´ from mixed infection of two viruses SLRSV and BRV and two clones of white currant cultivar ´Orion´ from mixed infection of two viruses BRV and GVBaV. Both clones of red currant cultivar ´Vitan´ remained infected with one of two viruses (BRV or GVBaV), which were detected in initial bush before the beginning of thermotherapy.
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