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Ambivalent sexism theory and techniques of its detection
Nováková, Veronika ; Smetáčková, Irena (advisor) ; Lukavská, Kateřina (referee)
This diploma thesis is dealing with the theory of ambivalent sexism and the technique of how to measure it. The main goal of the thesis is the conversion and validation of the 22 - item Ambivalent Sexism Inventory toward Women (ASI, Glick & Fiske, 1996) and through this inventory, the validity of the ambivalent sexism theory, from which the inventory stems from, on the Czech population. The thesis is therefore primarily focused on psychometric quality of the presented items and on the structure mapping of factors of the ASI in Czech setting. Secondarily it describes what the ASI says about Czech society. The conversion and validation of the ASI was made on a representative sample numbering 120 respondents in line with selection quotas. For the measurement of psychometric quality of the questions asked in the ASI, Cronbach's alfa was used as well as discriminability, skewness and kurtosis. Exploratory and confirmatory factor analyses were used for working with the factors and structure of the ASI. As for determining the content validity of the ASI, scenarios evaluated through Spearman's correlation coefficient with the included items, were employed. The results showed that the items of hostile sexism dispose of a psychometric quality unlike the items of benevolent sexism. The items dealing with...
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Cationic surface active compounds and evaluation of its properties
Pekařová, Kateřina ; Nováková, Veronika (advisor) ; Kuchařová, Monika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Consultant: PharmDr. Jan Marek, Ph.D. Supervisor: doc. PharmDr. Veronika Nováková, Ph.D. Student: Kateřina Pekařová Title of Thesis: Cationic surface active compounds and evaluation of its properties Quaternary ammonium salts are used as surfactants in many branches of the food, pharmaceutical and chemical industries. The basic properties of these substances were studied in the thesis. The critical micellar concentration of the homologous series of five substances was determined using the conductometric method. The theory of declining critical micellar concentration with the increasing length of the side alkyl chain has been verified. The hydrolytic activity of two commonly used cationic surfactants benzalconium chloride and benzoxonium chloride for the decomposition of the organophosphorus pesticide fenitrothion was tested. For these two substances, the effect of pH and temperature changes was evaluated. The influence of tenside concentration was also monitored. Benzoxonium chloride at 50 řC and pH 11was found as the most effective hydrolytic catalyst for the decomposition of the organophosphorus pesticide fenitrothione.
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Measurement of dissociation constants of potential antituberculotics
Suchý, Miroslav ; Kubíček, Vladimír (advisor) ; Nováková, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Miroslav Suchy Consultant: Ing. Vladimir Kubicek, CSc. Title of Thesis: Measurement of dissociation constants of potential antituberculotics This rigorous thesis deals with the measurement of dissociation constants substances newly synthesized at the Department of Pharmaceutical Chemistry and Pharmaceutical Analysis of the Faculty of Pharmacy of Charles University in Hradec Kralove. It is assumed that these substances could serve to treat tuberculosis disease.[1] The measurement was performed by potentiometric and spectrophotometric method. The match between the results of both methods is usually very good. Newly synthesized drugs are based on the structure of pyrazinamide and the measured values of dissociation constants should serve as supplementary information in further research.
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Synthesis of azaphthalocyanine quenchers of fluorescence for intrastrand binding to oligonucleotides
Machan, Matěj ; Nováková, Veronika (advisor) ; Miletín, Miroslav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmaceutical chemistry and Pharmaceutical analysis Candidate: Matěj Machan Supervisor: doc. PharmDr. Veronika Nováková, Ph.D. Title of thesis: Synthesis of novel azaphthalocyanines working as dark quenchers suitable for binding to the middle of an oligonucleotide chain Azaphthalocyanines (AzaPcs) are analogues of well-known phthalocyanines with benzene rings replaced for pyrazine ones. AzaPcs can be used in many applications due to their interesting spectral and photophysical properties, e.g. as photosensitisers in photodynamic therapy or as fluorescence sensors. The alkylamino substituted AzaPcs can be used as dark quenchers of fluorescence in mono-labeled DNA hybridization probes (FRET probes) or double-labeled probes as well. The AzaPcs for this application have typically three quarters identical (bearing alkylamino groups responsible for quenching of fluorescence) and the last quarter is modified with a functional group for attaching to oligonucleotide probe. The goal of this project was to synthesize unsymetrical AzaPc with a T-shape functional group carrying two hydroxy groups that can be used for attaching to oligonucleotide probe. The synthesis consisted of preparation of appropriately substituted...
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Synthesis and Study of New Unsymmetrical Azaphthalocyanines bearing azathiocrown ether for cation binding
Šeminská, Patrícia ; Nováková, Veronika (advisor) ; Miletín, Miroslav (referee)
1 ABSTRACT Charles University, Faculty of Pharmacy in Hradec Kralove Department: Department of Biophysics and Physical Chemistry Candidate: Patrícia Šeminská Supervisior: doc. PharmDr. Veronika Nováková, Ph.D. Consultant: PharmDr. Lukáš Lochman, Ph.D. Title of thesis: Synthesis and Study of New Unsymmetrical Azaphthalocyanines bearing azathiocrown ether for cation binding Azaphthalocyanines (AzaPc) are planar macrocyclic compounds, which are aza- analogues of synthetic phthalocyanine dyes. AzaPc, due to their system of conjugated double bonds, show interesting photophysical properties such as fluorescence emission and absorption in the red area of the spectrum, which is suitable for biological applications. These properties depend on character of peripheral substituents and central metal cation. The attachment of alkylamine group enables intramolecular charge transfer (ICT), that causes efficient quenching of fluorescence (non-fluorescent OFF state of the sensor), and leads to decrease of fluorescence quantum yield to zero. Blocking of ICT is used in development of new fluorescent sensors for metal cations, in which metal cation is coordinated into recognition moiety (e.g. azacrown ether) of the sensor. It results in a significant increase of fluorescence (fluorescent ON state of the sensor). Preparation of...
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Dietary prevention of type 2 diabetes mellitus
Nováková, Veronika ; Flekač, Milan (advisor) ; Mikeš, Ondřej (referee)
This thesis deals with dietary prevention of type 2 diabetes mellitus. The first chapters of the thesis deal with general information about diabetes mellitus. The chapters focus on the classification, epidemiology, clinical manifestation, complications, diagnostics of diabetes mellitus and pathogenesis of type 2 diabetes mellitus. The subsequent chapters describe prediabetes, obesity and metabolic syndrome. The conclusion of the theoretical section is devoted to the prevention of type 2 diabetes mellitus. The prevention is focused on non-pharmacological measures with an emphasis on dietary influences. The practical part is based on anonymous quantitative research in the form of an Internet quastionaire survey, which focuses on the prevention of type 2 diabetes mellitus. The main goal was to find out about the public's awareness of dietary effects on type 2 diabetes mellitus. The secondary objectives examine the awareness of the worlwide increase of diabetics and whether the respondents consider sugar to be the cause of type 2 diabetes mellitus and attach greater importance to healthy lifestyle or pharmacological measures in prevention. The survey results relating to dietary influences were not very satisfactory and respondents' poor awareness regarding prevention was reflected, for example in...
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Synthesis of azaphthalocyanine quenchers of fluorescence for intrastrand binding to oligonucleotides
Machan, Matěj ; Nováková, Veronika (advisor) ; Miletín, Miroslav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmaceutical chemistry and Pharmaceutical analysis Candidate: Matěj Machan Supervisor: doc. PharmDr. Veronika Nováková, Ph.D. Title of thesis: Synthesis of novel azaphthalocyanines working as dark quenchers suitable for binding to the middle of an oligonucleotide chain Azaphthalocyanines (AzaPcs) are analogues of well-known phthalocyanines with benzene rings replaced for pyrazine ones. AzaPcs can be used in many applications due to their interesting spectral and photophysical properties, e.g. as photosensitisers in photodynamic therapy or as fluorescence sensors. The alkylamino substituted AzaPcs can be used as dark quenchers of fluorescence in mono-labeled DNA hybridization probes (FRET probes) or double-labeled probes as well. The AzaPcs for this application have typically three quarters identical (bearing alkylamino groups responsible for quenching of fluorescence) and the last quarter is modified with a functional group for attaching to oligonucleotide probe. The goal of this project was to synthesize unsymetrical AzaPc with a T-shape functional group carrying two hydroxy groups that can be used for attaching to oligonucleotide probe. The synthesis consisted of preparation of appropriately substituted...
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Derivatives of quinoxaline-2-carboxylic acid as potential antimicrobial compounds
Bouz, Sarah ; Zitko, Jan (advisor) ; Nováková, Veronika (referee)
(ENGLISH) Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Sarah Basem Bouz Supervisor: PharmDr. Jan Zitko, Ph.D. Title of diploma thesis: Derivatives of quinoxaline-2-carboxylic acid as potential antimicrobial compounds Despite the presence of well-established treatment plan, tuberculosis remains the number one killer of infections according to WHO. One of the reasons behind this failure in eradicating this infection is drug resistance. This fact potentiates worldwide efforts to develop new antituberculars. As part of our long-term research on pyrazine derivatives, we prepared a series of N-substituted quinoxaline-2-carboxamides, refer to fig. below. Quinoxaline-2-carboxylic acid was activated by oxalyl chloride and reacted with different anilines or benzylamines in the presence of pyridine at room temperature, overnight with stirring, and then obtained crudes were purified with flash chromatography. Final products were evaluated for in vitro antimicrobial activities against six mycobacterial strains, eight fungal stems, along with four gram positive and four gram negative bacteria of clinical importance. The most promising compound among all with broad spectrum of antimycobacterial activity (MICMtbH37Ra = 3.91...
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Music Therapy and use for disabled children
NOVÁKOVÁ, Veronika
The thesis contains a theoretical and a practical part. The theoretical part deals with a concept and a definition of music therapy, history, aims, methods of music therapy and also organisations where music therapy is done. The thesis deals with connection of music therapy and children with disabilities. The topic of disability is described in several categories. It lists the types of disabilities, connection between disabled children and music therapy. It also deals with a funcion of a music therapist and it inroduces different therapists who work with us. The practical part contains my personal project, which consists of 3 lesson in length of 45 minutes each. I took advantage of the fact that in my surrounding there is a disabled child and I organized lessons of music therapy with him.
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Physico-Chemical Properties of Drugs
Tomanová, Pavla ; Kubíček, Vladimír (advisor) ; Nováková, Veronika (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of biophysics and physical chemistry Author: Pavla Tomanová Supervisor: Ing. Vladimír Kubíček, CSc. Thesis title: Physico-Chemical properties of drugs This thesis deals with measuring dissociation constants (the pKa value) and the lipophilicity of nitrogenous heterocycle derivatives produced at the Faculty of Pharmacy in Hradec Králové. Two methods were used to determine the pK value: potentiometric titration and spectrophotometry. Potentiometric titration almost invariably produced relatively exact results, however the results of pK value obtained by spectrophotometry were sometimes difficult to replicate. Dissociation constants were compared on the basis of the method applied, and also with the theoretically determined dissociation constant calculated by means of the ChemDraw Ultra 12.0 software. The results obtained by the two experimental methods turned out to be very similar, corresponding to the theoretical pK value. Lipophilicity is another essential property of drugs tested in the preclinical stage. It specifies the aqueous or lipophilic phase affinity of a drug, substantially affecting the drug's ADMET in organism. Lipophilicity was determined on the basis of capacity factor value identified by RP-HPLC measurement using...
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