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Czech Language on Tombstones in Iowa, USA
Měkotová, Barbora ; Dittmann, Robert (advisor) ; Eckertová, Eva (referee)
The aim of this thesis is to describe the Czech language on tombstones in selected cemeteries in the state of Iowa in the U.S., based on photos made in cooperation with Americans and photographs of Iowa cemeteries available on website. The first part deals with the emigration to the United States, the development of settlement Czech immigrants and Czech immigrant communities in Iowa. Then the text mentions the issue of epitaphs as evidence of assimilation of immigrants. Based on available data, examined photographs of tombstones are listed in Annex together with transcribed inscriptions on them. Following section involves linguistic analysis of texts, where is the influence of English reflected. The work is based on studies of E. Eckert, who devoted her work to the Czech language on the tombstones in Texas.
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Effect of tyrosine kinase inhibitor cabozantinib and cytotoxic alkaloid ellipticine on expression and activity of cytochromes P450 1A1, 1A2 and 1B1
Měkotová, Barbora ; Dračínská, Helena (advisor) ; Jeřábek, Petr (referee)
In recent years, tyrosine kinase inhibitors have been more and more used for the targeted cancer therapy, due to their ability to disrupt intracellular signalling pathways associated with the development of tumours. Cabozantinib is the tyrosine kinase inhibitor which has been approved for the treatment of thyroid cancer and it is also effective against several other types of cancer. However, multiple drugs combination is often used in anticancer therapy, which may result in their cytochrome P450-mediated interactions. Although this may affect the therapeutic effect of the drugs and cause adverse effects on the organism, very little is known about the effect of cabozantinib on biotransformation enzymes. Therefore, the effect of cabozantinib not only alone but also in combination with the known cytostatic ellipticine on the expression and the activity of cytochromes P450 1A1, 1A2 and 1B1 in rat liver and kidney in vivo was studied in this work. The gene expression was determined by quantitative PCR, the amount of protein was studied by Western blotting and consecutive immunodetection. The enzyme activity was studied using specific marker reactions, 7-ethoxyresorufin O-deethylation for CYP1A1, 7-methoxyresorufin O-demethylation for CYP1A2 and 17β-estradiol 4-hydroxylation for CYP1B1. Our results...
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The effects of endocrine disruptors on the expression and the activity of cytochromes P450 2B in laboratory rat as a model organism
Měkotová, Barbora ; Dračínská, Helena (advisor) ; Levová, Kateřina (referee)
Endocrine disrupting chemicals are compounds that interfere with natural hormones and they are responsible for functional changes which may lead to damage of the endocrine system. Their presence in the environment is associated with a number of diseases whose extent is hard to predict. As endocrine disrupting chemicals, a wide range of exogenous and endogenous compounds is present in the environment. Important exogenous endocrine disrupting chemicals include benzo[a]pyrene (BaP) and 17α-ethinylestradiol (EE2); the female sex hormone 17β-estradiol (E2) can act like endogenous endocrine disruptor. In this thesis, the effect of these three compounds and their combinations on the expression and the activity of rat biotransformation enzymes cytochromes P450 2B is studied. The gene expression was determined by quantitative PCR, the expression of the protein itself was studied using Western blot method and consecutive immunodetection. The results show that CYP2B expression is almost unchanged after BaP premedication, whereas estrogenic compounds, E2, EE2, their combination and their combinations with BaP, significantly decrease the expression. The enzyme activity of CYP2B was also studied in rat liver microsomes using the marker substrate 7-pentoxyresorufin. EE2, E2 and their combination decrease the...
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Czech Language on Tombstones in Iowa, USA
Měkotová, Barbora ; Dittmann, Robert (advisor) ; Eckertová, Eva (referee)
The aim of this thesis is to describe the Czech language on tombstones in selected cemeteries in the state of Iowa in the U.S., based on photos made in cooperation with Americans and photographs of Iowa cemeteries available on website. The first part deals with the emigration to the United States, the development of settlement Czech immigrants and Czech immigrant communities in Iowa. Then the text mentions the issue of epitaphs as evidence of assimilation of immigrants. Based on available data, examined photographs of tombstones are listed in Annex together with transcribed inscriptions on them. Following section involves linguistic analysis of texts, where is the influence of English reflected. The work is based on studies of E. Eckert, who devoted her work to the Czech language on the tombstones in Texas.
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