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The Study of the Immune Response of Larvae of the Fleshfly Sarcophaga Bullata
Mášová, Alice ; Jiráček, Jiří (advisor) ; Žurovec, Michal (referee) ; Bezouška, Karel (referee)
Conclusion lnsect immune response is a complicated process. Antimicrobiď peptides fiequently act synergistically, some proteins have transporter functions, peptide inhibitors can block processing enzyrnes involved in activating cascades or different enzymes can activarc antimicrobially active proteins or peptides. In this study we presented nvo different isolation protocols, which resulted in the identification of several already known and two novel antirnierobial proteins or peptides from the hemolymph of the larvae of flesbfty. sareophaga bullan. We are the first group to monitor the timecourse of tissue-specific expÍession patlems of eight genes in Sarcophaga bullan |arvae ďter different immune challenges using qPCR. we show similarities, as well as differences in insect immune responses. we are also the first group to analyze the expression pattoms of the genes that encode sBp and sarcocystatin, proteins that are mainly connect€d with larvat development and metamorphosis. Using 2D- electrophoresis we analyzď the time.dependent immune response in larval fat bodies and hemocytes. We detect€d 9 up.regulated proteins in hemocyŮes and 15 differentially expressed proteins in fat body cells. We hope that our study will shed more light into ttre complex processes of immune responses in Sarcophaga bullan larvae. 20
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The Study of the Immune Response of Larvae of the Fleshfly Sarcophaga Bullata
Mášová, Alice ; Jiráček, Jiří (advisor) ; Žurovec, Michal (referee) ; Bezouška, Karel (referee)
Conclusion lnsect immune response is a complicated process. Antimicrobiď peptides fiequently act synergistically, some proteins have transporter functions, peptide inhibitors can block processing enzyrnes involved in activating cascades or different enzymes can activarc antimicrobially active proteins or peptides. In this study we presented nvo different isolation protocols, which resulted in the identification of several already known and two novel antirnierobial proteins or peptides from the hemolymph of the larvae of flesbfty. sareophaga bullan. We are the first group to monitor the time<ourse of tissue-specific expÍession patlems of eight genes in Sarcophaga bullan |arvae ďter different immune challenges using qPCR. we show similarities, as well as differences in insect immune responses. we are also the first group to analyze the expression pattoms of the genes that encode sBp and sarcocystatin, proteins that are mainly connect€d with larvat development and metamorphosis. Using 2D- electrophoresis we analyzď the time.dependent immune response in larval fat bodies and hemocytes. We detect€d 9 up.regulated proteins in hemocyŮes and 15 differentially expressed proteins in fat body cells. We hope that our study will shed more light into ttre complex processes of immune responses in Sarcophaga bullan larvae. 20
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Chiral analysis of beta-alanyl-D,L-tyrosine and its derivatives by capillary electrophoresis with 2-hydroxypropyl-beta-cyclodextrin stereoselector
Sázelová, Petra ; Šolínová, Veronika ; Schimperková, Tereza ; Mášová, Alice ; Jiráček, Jiří ; Kašička, Václav
A new capillary electrophoretic method was developed for separation of enantiomers of antimicrobial dipeptide betha-Ala-D,L-Tyr and its derivatives using 50 mM Tris-phosphate, pH 2.50, as background electrolyte and 2-hydroxypropyl-betha-cyclodextrin at concentrations 20 – 60 mg/mL as chiral selector. In addition, association constants of complexes of the enatiomers of dipeptide betha-Ala-D,L-Tyr and its derivatives with 2-hydroxypropyl-betha-cyclodextrin chiral selector were determined.
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