|
|
Effect of the peptides on osteogenic differentiation of mesenchymal steam cells
Lukášová, Věra
Osteogenic differentiation of mesenchymal stem cells (MSCs) would be possible to induce by creating of a cell bioactive scaffold that mimic the properties of bone extracellular matrix (ECM). This induction will be not only due to the addition of osteogenic supplements, but also due to the addition of differentiation peptides. These peptides activate signaling pathways leading to cell differentiation. The aim of this study was to evaluate the effect of selected peptides on adhesion, metabolic activity, proliferation and osteogenic differentiation of porcine MSCs. Four peptides with amino acid sequences of DGEA, IAGVGGEKSGGF, GQGFSYPYKAVFSTQ and KIPKASSVPTELSAISTLYL were selected. These peptides were derived from receptor binding sequences of collagen I, collagen III, BMP-7 and BMP-2 respectively. Scaffolds were prepared from a biocompatible and biodegradable poly-ε-caprolactone (PCL) polymer, suitable for cell cultivation. Cells were cultured on scaffolds for three weeks. Various concentration of differentiation peptides were added to the culture medium. As observed in the experiment of cells cultured in basal medium supplemented with differentiation peptides no effect on adhesion, proliferation or metabolic activity of porcine MSCs was observed. In groups treated with peptides derived from BMP-2...
|
|
|
Cell-free scaffolds functionalized with bioactive compounds in bone tissue engineering
Lukášová, Věra ; Rampichová, Michala (advisor) ; Hubálek Kalbáčová, Marie (referee) ; Libusová, Lenka (referee)
There are good prospects for bone tissue engineering and therefore researcher is aimed towards the development of cell-free scaffolds. A cell-free scaffold serves as a temporal filling for critical size defects that do not heal spontaneously. Nevertheless, a suitable scaffold composition is yet to be discovered. Moreover, modifications of cell-free scaffolds with a drug delivery system activate the internal healing capacity. Platelets occur in the healing cascade as a natural source of growth factors (GFs), chemokines and cytokines. This autologous source of bioactive compounds enables the substitution of synthetic GFs. The aim of this thesis is to develop a bioactive cell-free scaffold with a drug delivery system supporting the physiological healing of bone defects. The centrifugal spinning method was used to produce nanofibrous poly-ε-caprolactone (PCL) scaffolds. PCL scaffolds were functionalized with different platelet concentrations. Bioactive compounds released from activated platelets were trapped within the formed fibrin net, enabling their gradual release. Improved metabolic activity, proliferation and alkaline phosphatase activity of MG-63 cells and human mesenchymal stem cells (hMSCs) were detected. The release of compounds lasted for two weeks and nearly reached the plateau phase,...
|
|
|
Effect of the peptides on osteogenic differentiation of mesenchymal steam cells
Lukášová, Věra
Osteogenic differentiation of mesenchymal stem cells (MSCs) would be possible to induce by creating of a cell bioactive scaffold that mimic the properties of bone extracellular matrix (ECM). This induction will be not only due to the addition of osteogenic supplements, but also due to the addition of differentiation peptides. These peptides activate signaling pathways leading to cell differentiation. The aim of this study was to evaluate the effect of selected peptides on adhesion, metabolic activity, proliferation and osteogenic differentiation of porcine MSCs. Four peptides with amino acid sequences of DGEA, IAGVGGEKSGGF, GQGFSYPYKAVFSTQ and KIPKASSVPTELSAISTLYL were selected. These peptides were derived from receptor binding sequences of collagen I, collagen III, BMP-7 and BMP-2 respectively. Scaffolds were prepared from a biocompatible and biodegradable poly-ε-caprolactone (PCL) polymer, suitable for cell cultivation. Cells were cultured on scaffolds for three weeks. Various concentration of differentiation peptides were added to the culture medium. As observed in the experiment of cells cultured in basal medium supplemented with differentiation peptides no effect on adhesion, proliferation or metabolic activity of porcine MSCs was observed. In groups treated with peptides derived from BMP-2...
|
|
|
Effect of the peptides on osteogenic differentiation of mesenchymal steam cells
Lukášová, Věra ; Amler, Evžen (advisor) ; Filová, Elena (referee)
Osteogenic differentiation of mesenchymal stem cells (MSCs) would be possible to induce by creating of a cell bioactive scaffold that mimic the properties of bone extracellular matrix (ECM). This induction will be not only due to the addition of osteogenic supplements, but also due to the addition of differentiation peptides. These peptides activate signaling pathways leading to cell differentiation. The aim of this study was to evaluate the effect of selected peptides on adhesion, metabolic activity, proliferation and osteogenic differentiation of porcine MSCs. Four peptides with amino acid sequences of DGEA, IAGVGGEKSGGF, GQGFSYPYKAVFSTQ and KIPKASSVPTELSAISTLYL were selected. These peptides were derived from receptor binding sequences of collagen I, collagen III, BMP-7 and BMP-2 respectively. Scaffolds were prepared from a biocompatible and biodegradable poly-ε-caprolactone (PCL) polymer, suitable for cell cultivation. Cells were cultured on scaffolds for three weeks. Various concentration of differentiation peptides were added to the culture medium. As observed in the experiment of cells cultured in basal medium supplemented with differentiation peptides no effect on adhesion, proliferation or metabolic activity of porcine MSCs was observed. In groups treated with peptides derived from BMP-2...
|
|
|
Effect of plasma modified nanofiber surface on mesenchymal stem cell adhesion, viability and proliferation.
Lukášová, Věra ; Amler, Evžen (advisor) ; Svoboda, Ondřej (referee)
This work summarizes information about the interactions between the plasma modified nanofibres surfaces and mesenchymal stem cells. Knowledge of mechanisms of cell specific adhesion could be used for a development of artificial scaffolds which will support a growth of the particular cell type. Nanofibrous materials mimic the morphology of the extracellular matrix and serve as cell scaffolding materials. However, the cell adhesion to the material surface is often not sufficient for efficient tissue regeneration. Plasma modification of nanofiber scaffolds leads to an improvement of surface characteristics. Changes in wettability, surface energy, roughness and chemical composition occur in various extents according to the type of utilized plasma. Such modifications could lead to improvement of cell adhesion and could enhance the potential of scaffold application for biomedical use.
|
|
|
Position of the head sister viewed by a medical sttaf member
LUKÁŠOVÁ, Věra
The position of the head sister viewed by a medical staff member My thesis examines the position of the head sister from the viewpoint of staff nurses both in state-run and private health care facilities. It gives insight into contacts between the head sister and staff nurses, especially if staff nurses are informed enough about the head sister´s duties. The results obtained in state-run and private health care facilites are compared. The following hypotheses were set: H I - staff nurses get into a personal contact with the head sister at least twice a year; H II {--} staff nurses are informed about the duties of the head sister ; H III {--} staff nurses working in hospitals, Inc., get into personal contacts with the head sister more often than staff nurses working in teaching hospitals. I set up thirty questions to address the issues of the position of the head sister in the hospital management and her duties. To obtain as much as possible information and to involve as many as possible respondents, the quantitative method of a questionnaire was used. The questionnaire consisted mainly of closed and semi-closed questions .One open question was also included to enable the respondents to express their own opinions. Respondents from the teaching hospital in Plzen and several other hospitals in the Plzen region ( the hospital Primaved, the Mulac hospital, the hospital in Stod, the hospital in Domazlice) took part in the investigation. The results of the investigation were processed in two phases: first the overall summary of all the replies was made and in the second phase the replies of the particular groups of respondents were evaluated separately. The investigation shows that staff nurses come into contact with the head sister and are familiar with her duties. The same conclusion was reached by comparing the replies obtained from staff nurses working in both kinds of hospitals, state-run and private. The results suggest that stuff nurses in both kinds of hospitals are thus well informed about the position of thehead sister. The objective of the investigation was achieved. The hypotheses I and II were confirmed, the hypohtesis III was not confirmed. The conclusions of the investigation will be presented at a workshop in the teaching hospital in Plzen with the approval of the head sister.. If the medical periodical is interested the conclusions will also be published there.
|
guest ::
RSS feed.