National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Neuronal cell culture in vitro
Kohoutová, Šárka ; Bařinka, Cyril (advisor) ; Pavlíček, Jiří (referee)
Neuronal cell cultures are in vitro cultures of dissociated neurons that have become an essential part of many neurobiological experiments in the last century. Cultured neurons not only allow to answer questions about their physiology under complex in vivo conditions, but also can serve as a model of neurodegenerative diseases. Neuronal cells can either be isolated directly from the nervous tissue of animals at the prenatal or adult stage of development, or they can be obtained through targeted manipulations of stem cells and secondary cell lines that lead to their neuronal differentiation. Primary neurons are considered the gold standard of neurobiological research not only because of their long tradition of cultivation, but also because primary neurons retain typical neuronal properties under in vivo conditions. There are several disadvantages associated with primary neurons, including the fact that fully differentiated neurons do not proliferate and are relatively demanding in terms of culture conditions For this reason, their role is often replaced by mitotically active secondary cell lines or stem cells. This bachelor thesis summarizes the knowledge about cell cultures used to study the functions of neuronal cells and highlights the advantages and disadvantages of their use. Key words Primary...
Chocolate and other Chosen Sweets in Nutrition of Children
Kohoutová, Šárka ; Marádová, Eva (advisor) ; Vodáková, Jitka (referee)
My project is concentrated on the problems of sweets in nourishment. In my project I deal with the question how the health of children is influenced by eating sweets, especially the eating of chocolate and ice-cream. In the theoretical part I summarize the information about sweets and its influence on the health condition. The goal of the practical part is trying to find out more about preference and consuming of sweets among teenagers. Powered by TCPDF (www.tcpdf.org)

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