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Identification of purified biotransformation enzymes by means of mass spectrometry
Křížová, Andrea ; Lenčo, Juraj (advisor) ; Řehulka, Pavel (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Andrea Křížová Supervisor: PharmDr. Juraj Lenčo, Ph.D Title of diploma thesis: Identification of purified biotransformation enzymes by means of mass spektrometry Biotransformation enzymes are involved in the metabolism of a wide range of both endogenous compounds and xenobiotics. Regarding the metabolism of xenobiotics, many of those enzymes have not been characterized at all or only marginally. To get better knowledge about how the biotransformation enzymes affect compounds with significant impact on the organism as a whole, they need to be purified, identified and subsequently thoroughly characterized. The classical electrophoresis is still irreplaceable for protein purification and characterization. Moreover, combination of electrophoresis with mass spectrometry (MS) represents an inseparable tandem for protein identification. However, to obtain unambiguous outputs from mass-spectrometry based protein identification, it is essential to avoid unwanted contamination of the sample during sample preparation. Wearing disposable protective equipments, using MS grade chemicals, solutions filtering are examples of rules for sample preparation for MS analysis that were established at the...
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Identification of purified biotransformation enzymes by means of mass spectrometry
Křížová, Andrea ; Lenčo, Juraj (advisor) ; Řehulka, Pavel (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Andrea Křížová Supervisor: PharmDr. Juraj Lenčo, Ph.D Title of diploma thesis: Identification of purified biotransformation enzymes by means of mass spektrometry Biotransformation enzymes are involved in the metabolism of a wide range of both endogenous compounds and xenobiotics. Regarding the metabolism of xenobiotics, many of those enzymes have not been characterized at all or only marginally. To get better knowledge about how the biotransformation enzymes affect compounds with significant impact on the organism as a whole, they need to be purified, identified and subsequently thoroughly characterized. The classical electrophoresis is still irreplaceable for protein purification and characterization. Moreover, combination of electrophoresis with mass spectrometry (MS) represents an inseparable tandem for protein identification. However, to obtain unambiguous outputs from mass-spectrometry based protein identification, it is essential to avoid unwanted contamination of the sample during sample preparation. Wearing disposable protective equipments, using MS grade chemicals, solutions filtering are examples of rules for sample preparation for MS analysis that were established at the...
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The importance of lookout towers for tourism development in Central Bohemia Region
Křížová, Andrea ; Netková, Jarmila (advisor) ; Petrů, Zdenka (referee)
The central theme of the thesis are look-out towers and their use in tourism. The thesis is divided into four main sections that follow the content itself. The opening chapter deals with the general definition of tourism industry and current trends of traveling in the Czech Republic. The following chapter is focused on a selected region of the Central Bohemia in terms of status and differences in the travel industry. The third part focuses on the historical development, construction of look-out towers and a description of the most famous towers in the Central Bohemia. The attractiveness of 23 selected observation towers and their potential for development and growth of tourism was examined in the practical part of the thesis. The analysis evaluates the observation towers in light of technical parameters, construction, quality of service in the area, information system and promotion. To illustrate the current situation of lookouts in the Czech Republic was connected the opinion of expert for towers Jan Nouza (co-authored a famous paper, "Man, look around"), which is expressed by means of a questionnaire to the specific deficiencies and the importance of towers in the Czech Republic.
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