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Molecular mechanisms of the assembly and function of BBSome
Prasai, Avishek ; Huranová, Martina (advisor) ; Varga, Vladimír (referee) ; Bosáková, Michaela (referee)
Bardet Biedl syndrome is a genetic disorder caused by the dysfunction of the BBSome, an octameric cargo adaptor protein complex. The BBSome facilitates the transport of signaling receptors into and out of the primary cilium, a microtubule based sensory organelle of the cell. The first part of this thesis focuses on the elucidation of the assembly of the BBSome in living cells. We generated a library of human and mouse cells lines deficient in the individual BBSome subunits and transduced them with the other YFP tagged subunits. We employed biochemical assays, immunofluorescence and quantitative fluorescence microscopy techniques to analyze the individual steps in the BBSome assembly pathway. We revealed that the BBSome assembly occurs sequentially in spatially regulated steps. We showed that BBS4 nucleates the assembly of a pre-BBSome at the pericentriolar satellites. The translocation of the pre-BBSome to the ciliary base is facilitated by BBS1. We also revealed that in a BBS chaperonin deficient cell line, BBS12 KO cells, a small fraction of the BBSome and/or BBSome sub-complexes are still able to form and localize to the cilium. This could suggest that the BBS chaperonins might act later in the BBSome assembly pathway providing a means for quality control for the BBSome. Ciliary ectocytosis...
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The effect of "neutral player" in the small-sided-game 4 on 4 in handball
Huráňová, Martina ; Tůma, Martin (advisor) ; Hráský, Pavel (referee)
Title: The effect of "neutral player" in the small-sided game 4 on 4 in handball Objectives: The main objective of this work is to analyze the characteristics of internal and external loads. The load is analyzed in the framework of small-sided games in team handball for women DHK Zora Olomouc. Diploma thesis partially continues and develops the work of bachelor Intensity loads of players in modifications handball from 2013 and this work is developing in many ways. Small-sided games are extended by "neutral player" and compared the effect of this player in individual games. For deciding whether the effect of "neutral player" significant or not it is used test statistics. Methods: In this thesis, the method of observation to track phenomena that are directly available to sensory perception. Furthermore, the method of questioning when based on forward-known indicators survey participants are asked about their assessment. The acquisition values of heart rate is used meter Polar Team 2. Finally, the method of data analysis for examining and identifying relationships between the data obtained. Results: During this study it was found that the involvement of "neutral players" in small-sided games is statistically insignificant. It does not contribute to the improvement of physical fitness. The results were...
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