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Determination of double bond position in wax esters by dimethyl disulfide derivatization and mass spectrometry
Háková, Martina ; Schwarzová, Karolina (advisor) ; Coufal, Pavel (referee)
Wax esters are substantial constituents of natural waxes, which can be found in many living organisms. Properties of lipids, including wax esters, may be significantly influenced by the position of double bond. In this diploma thesis the location of double bonds was determined by dimethyl disulfide (DMDS) derivatization followed by detection using tandem mass spectrometry with atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI). We managed to measure the APCI and ESI MS/MS spectra of 8 different wax esters with different position of double bond. Diagnostic ions determining double bond position were identified. This method could be used in HPLC/MS analysis of wax esters, which cannot be analyzed by GC/MS. It was shown that the DMDS derivatization reaction and mass detection with APCI ionization is also suitable for locating double bonds in alkenes.
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Application of nanofibrous sorbents for on-line extraction in liquid chromatography
Háková, Martina ; Šatínský, Dalibor (advisor) ; Česla, Petr (referee) ; Urban, Jiří (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Martina Háková Supervisor: doc. RNDr. Dalibor Šatínský, Ph.D. Consultant: doc. PharmDr. Lucie Chocholoušová Havlíková, Ph.D. Title of Doctoral Thesis: Application of nanofibrous sorbents for on-line extraction in liquid chromatography A compilation of ten publications is presented in this dissertation thesis. It is focused on the application of nano and microfibrous polymers as a new extracting material in analytical chemistry. This work was based on close cooperation with the Institute for Nanomaterials, Advanced Technologies and Innovation and the Department of Nonwovens and Nanofibrous Materials at the Technical University of Liberec, where the author took part in designing and preparation of these materials. The first part deals with model studies, based on using polyamide, polystyrene and polycaprolactone nanofibers as sorbents for solid phase extraction in classical off-line mode or in on-line column switching system. It solves practical aspects such as orientation and the formation of the fibers in the extraction system, optimization of fabrication factors affecting applicability of the nanomaterials, suitable solvent systems, stability testing and reusability of the microcolumns. The...
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Application of nanofibrous sorbents for on-line extraction in liquid chromatography
Háková, Martina ; Šatínský, Dalibor (advisor) ; Česla, Petr (referee) ; Urban, Jiří (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Martina Háková Supervisor: doc. RNDr. Dalibor Šatínský, Ph.D. Consultant: doc. PharmDr. Lucie Chocholoušová Havlíková, Ph.D. Title of Doctoral Thesis: Application of nanofibrous sorbents for on-line extraction in liquid chromatography A compilation of ten publications is presented in this dissertation thesis. It is focused on the application of nano and microfibrous polymers as a new extracting material in analytical chemistry. This work was based on close cooperation with the Institute for Nanomaterials, Advanced Technologies and Innovation and the Department of Nonwovens and Nanofibrous Materials at the Technical University of Liberec, where the author took part in designing and preparation of these materials. The first part deals with model studies, based on using polyamide, polystyrene and polycaprolactone nanofibers as sorbents for solid phase extraction in classical off-line mode or in on-line column switching system. It solves practical aspects such as orientation and the formation of the fibers in the extraction system, optimization of fabrication factors affecting applicability of the nanomaterials, suitable solvent systems, stability testing and reusability of the microcolumns. The...
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Application of nanofibrous sorbents for on-line extraction in liquid chromatography
Háková, Martina ; Šatínský, Dalibor (advisor) ; Česla, Petr (referee) ; Urban, Jiří (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Martina Háková Supervisor: doc. RNDr. Dalibor Šatínský, Ph.D. Consultant: doc. PharmDr. Lucie Chocholoušová Havlíková, Ph.D. Title of Doctoral Thesis: Application of nanofibrous sorbents for on-line extraction in liquid chromatography A compilation of ten publications is presented in this dissertation thesis. It is focused on the application of nano and microfibrous polymers as a new extracting material in analytical chemistry. This work was based on close cooperation with the Institute for Nanomaterials, Advanced Technologies and Innovation and the Department of Nonwovens and Nanofibrous Materials at the Technical University of Liberec, where the author took part in designing and preparation of these materials. The first part deals with model studies, based on using polyamide, polystyrene and polycaprolactone nanofibers as sorbents for solid phase extraction in classical off-line mode or in on-line column switching system. It solves practical aspects such as orientation and the formation of the fibers in the extraction system, optimization of fabrication factors affecting applicability of the nanomaterials, suitable solvent systems, stability testing and reusability of the microcolumns. The...
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Determination of double bond position in wax esters by dimethyl disulfide derivatization and mass spectrometry
Háková, Martina ; Schwarzová, Karolina (advisor) ; Coufal, Pavel (referee)
Wax esters are substantial constituents of natural waxes, which can be found in many living organisms. Properties of lipids, including wax esters, may be significantly influenced by the position of double bond. In this diploma thesis the location of double bonds was determined by dimethyl disulfide (DMDS) derivatization followed by detection using tandem mass spectrometry with atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI). We managed to measure the APCI and ESI MS/MS spectra of 8 different wax esters with different position of double bond. Diagnostic ions determining double bond position were identified. This method could be used in HPLC/MS analysis of wax esters, which cannot be analyzed by GC/MS. It was shown that the DMDS derivatization reaction and mass detection with APCI ionization is also suitable for locating double bonds in alkenes.
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