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Business Plan of the Active Studio Development
Šanderová, Hana ; Jelínek, Jiří (referee) ; Šimberová, Iveta (advisor)
The master´s thesis deals with processing of business plan for the establishment of the active studio. The final prospectus goes from the complete analysis of the market in term of potential customers and also of current competition. The aim of the thesis is to make a survey to find out how feasible is the business plan. In graduation theses also I valorize diversification of the project.
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Intracellular dinucleoside polyphosphates and methods of their detection
Říha, Jan ; Macíčková Cahová, Hana (advisor) ; Šanderová, Hana (referee)
(EN) Dinucleoside polyphosphates are already known for more than 50 years, but their role inside a cell is still unclear. Some theories discuss their possible role as alarmones during stress conditions, others connect them to DNA damage or proliferation. One new theory is that dinucleoside polyphosphates act as 5' RNA caps. To elucidate their role in organisms, it is important to know their concentration in normal and stress conditions. This work will try to determine basal concentration in both bacterial and eukaryotic cells, and the changes of their concentration under stress conditions, from already known data. Measurement of concentration of any compound inside a cell depends on the used method. I also present basic overview of methods for detecting dinucleoside polyphosphates, from older luciferase- based techniques to new precise mass spectrometry-based techniques. Keywords: dinucleoside polyphosphates, Ap4A, RNA caps, cellular stress, LC-MS detection and quantification
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EF-Tu PROTEIN DOMAINS Functions and Thermostability
Šanderová, Hana ; Jonák, Jiří (advisor) ; Pichová, Iva (referee) ; Mikulík, Karel (referee)
6 % The bacterial elongation factor Tu (EF-Tu) has been extensively studied for decades as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in structure and function makes EF-Tu proteins suitable for the studies of evolutionary relationships between organisms and for elucidation of the structural features of adaptation to various living conditions. Furthermore, since all known EF-Tu proteins are composed of three distinct domains, they can also serve as suitable models for the understanding of domain organization in proteins. One of the main research projects of the Department of Gene Expression at the Institute of Molecular Genetics AS CR, where I did my PhD studies, was the study of the primary structure, transcription regulation and functions of bacterial elongation factors Tu from Gram positive thermophilic bacterium Bacillus stearothermophilus and from Gram negative mesophilic bacterium Escherichia coli. In this work, we focused on the structure-function relationships between EF-Tu proteins and their domains. The domain effect had been before our studies tested mainly by truncated EF-Tu forms lacking one or two domains. In contrast, we decided to...
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EF-Tu Protein Domains:Functions and Thermostability
Šanderová, Hana
Charles University in Prague Faculty of Science Department of Genetics and Microbiology EF-Tu PROTEIN DOMAINS Functions and Thermostability PhD thesis Hana Šanderová Specialization: Molecular and Cellular Biology, Genetics and Virology Supervisor: Prof. MUDr. Jiří Jonák, DrSc. Department of Gene Expression, Institute of Molecular Genetics AS CR Prague 2008 Summary of PhD Thesis The bacterial elongation factor Tu (EF-Tu) has been extensively studied for decades as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in structure and function makes EF-Tu proteins suitable for the studies of evolutionary relationships between organisms and for elucidation of the structural features of adaptation to various living conditions. Furthermore, since all known EF-Tu proteins are composed of three distinct domains, they can also serve as suitable models for the understanding of domain organization in proteins. One of the main research projects of the Department of Gene Expression at the Institute of Molecular Genetics AS CR, where I did my PhD studies, was the study of the primary structure, transcription regulation and functions of bacterial elongation factors Tu...
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Functional analysis of eIF3e subunit of human translation initiation factor 3 in living cells.
Šikrová, Darina ; Valášek, Leoš (advisor) ; Šanderová, Hana (referee)
2 Abstract Eukaryotic initiation factor 3 (eIF3) is a critical player involved in many steps of translation initiation, which ultimately result in the formation of the elongation competent 80S ribosome. With its 13 subunits (eIF3a - eIF3m) it is the largest and the most complex translation initiation factor composed of three mutually interconnected modules (i - iii), however, the role of individual subunits involved in its structural integrity and proper function is not fully explored. The eIF3e subunit was shown to be a part of the human eIF3 structural core and to help in the mRNA recruitment to the 43S pre-initiation complex by forming a molecular bridge between the 40S ribosomal subunit and the mRNA cap-binding complex. In this study, we employed siRNA-directed downregulation of eIF3e in HeLa cells and analysed its impact on the overall eIF3 integrity and function in vivo. The eIF3e knock-down (eIF3eK.D. ) led to the severe reduction of protein amounts of other three subunits (eIF3d, k and l), which together with the subunit eIF3c and e form module ii of the eIF3 complex. Remaining module i (composed of a, b, g and i) and iii (containing f, h and m) stayed partially bound perhaps thanks to a bridging effect of eIF3c, and showed reduced binding efficiency towards the 40S subunit compared to control...
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EF-Tu Protein Domains:Functions and Thermostability
Šanderová, Hana
Charles University in Prague Faculty of Science Department of Genetics and Microbiology EF-Tu PROTEIN DOMAINS Functions and Thermostability PhD thesis Hana Šanderová Specialization: Molecular and Cellular Biology, Genetics and Virology Supervisor: Prof. MUDr. Jiří Jonák, DrSc. Department of Gene Expression, Institute of Molecular Genetics AS CR Prague 2008 Summary of PhD Thesis The bacterial elongation factor Tu (EF-Tu) has been extensively studied for decades as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in structure and function makes EF-Tu proteins suitable for the studies of evolutionary relationships between organisms and for elucidation of the structural features of adaptation to various living conditions. Furthermore, since all known EF-Tu proteins are composed of three distinct domains, they can also serve as suitable models for the understanding of domain organization in proteins. One of the main research projects of the Department of Gene Expression at the Institute of Molecular Genetics AS CR, where I did my PhD studies, was the study of the primary structure, transcription regulation and functions of bacterial elongation factors Tu...
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EF-Tu PROTEIN DOMAINS Functions and Thermostability
Šanderová, Hana ; Jonák, Jiří (advisor) ; Pichová, Iva (referee) ; Mikulík, Karel (referee)
6 % The bacterial elongation factor Tu (EF-Tu) has been extensively studied for decades as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in structure and function makes EF-Tu proteins suitable for the studies of evolutionary relationships between organisms and for elucidation of the structural features of adaptation to various living conditions. Furthermore, since all known EF-Tu proteins are composed of three distinct domains, they can also serve as suitable models for the understanding of domain organization in proteins. One of the main research projects of the Department of Gene Expression at the Institute of Molecular Genetics AS CR, where I did my PhD studies, was the study of the primary structure, transcription regulation and functions of bacterial elongation factors Tu from Gram positive thermophilic bacterium Bacillus stearothermophilus and from Gram negative mesophilic bacterium Escherichia coli. In this work, we focused on the structure-function relationships between EF-Tu proteins and their domains. The domain effect had been before our studies tested mainly by truncated EF-Tu forms lacking one or two domains. In contrast, we decided to...
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Business Plan of the Active Studio Development
Šanderová, Hana ; Jelínek, Jiří (referee) ; Šimberová, Iveta (advisor)
The master´s thesis deals with processing of business plan for the establishment of the active studio. The final prospectus goes from the complete analysis of the market in term of potential customers and also of current competition. The aim of the thesis is to make a survey to find out how feasible is the business plan. In graduation theses also I valorize diversification of the project.
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