National Repository of Grey Literature 78 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
In vitro screening of novel potentially active antibacterial compounds II
Zbořilová, Eliška ; Janďourek, Ondřej (advisor) ; Vejsová, Marcela (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Bc. Eliška Zbořilová Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of novel potentially active antibacterial compounds II Key words: antibiotics, bacteria, resistance, minimal inhibitory concentration, bacterial susceptibility to antibiotics This diploma thesis deals with testing of the efficacy of new antimicrobial agents on selected representative bacteria. The work consists of a theoretical part and a practical part. The theoretical part includes the history and the present of antibiotics describing current issues and the layout of the antibiotic policy in the Czech Republic and in the world. It deals with the characteristics of drugs, below which are described their importance, division, effects, resistance. Furthermore, this part of work describes detailed mechanism of action for two selected groups of compounds. Last but not least, this work portrays the methods that are used to determine the sensitivity of bacteria to antibiotics. The practical part deals with the description of the whole work process, including the characteristics of used bacterial strains and the list of tested substances....
Listeria sp.
Králíková, Lenka ; Vejsová, Marcela (advisor) ; Janďourek, Ondřej (referee)
Author: Lenka Králíková Title: Listeria spp. Bachelor thesis Charles University in Prague, Faculty of Pharmacy, Hradec Králové Field of study: Medical Labolatory Background: The bachelor thesis aims to describe Listeria spp. Describe the division of genus, morphology and laboratory diagnostics. More attention is paid to Listeria monocytogenes as it is a major source of infection in humans. Another aim is to describe the occurrence of L. monocytogenes in food, its antigenic structure, pathogenesis and the disease it causes. Main findings: Listeria monocytogenes is a bacterium present everywhere. It was found in fresh water, clay, plant material and waste water. The main source of infection for humans is contaminated food. They are most often found in unpasteurized milk, matured cheeses or poorly cooked meat or vegetables. Bacteria causes a disease called listeriosis, which is especially dangerous for newborns, elderly people, pregnant women and immunocompromised individuals. The disease occurs rather sporadically. There was confirmed from 30 to 37 cases per year in the Czech Republic between years 2008-2017. There was also an epidemic in Africa in 2017-2018, it was the largest epidemic in the world. In the event of infection, both the laboratory and the physician are obliged to report the positivity...
Overview of methodological approaches leading to qualitative anad quantitative microbial biofilm evaluation
Holmanová, Pavlína ; Konečná, Klára (advisor) ; Vejsová, Marcela (referee)
Charles Univerzity Faculty of Pharmacy in Hradec Králové Study program: Medical Laboratory Technician Author: Pavlína Holmanová Supervisor: RNDr. Klára Konečná, Ph.D. Title of bachelor thesis: Overview of methodological approaches leading to qualitative and quantitative microbial biofilm evaluation Background: The aim of this work was to compose an overview of methodical approaches leading to qualitative and quantitative evaluation of microbial biofilms. To optimize the methods leading to preparation of mounts with biofilm-forming microorganisms evaluated by epifluorescence or confocal laser scanning microscopy. Methods: Optimization of microbial biofilm staining procedures for subsequent evaluation by epifluorescence microscopy. Formation of microbial biofilms - cultivation of microorganisms in various cultivation media in a microtiter plate, preparation of solutions with fluorescent dyes. Observation of coloured, dispersed biofilms by fluorescence microscope. Results: Using fluorescence microscopy, optimal target concentrations of fluorescent dyes in various combinations were verified. The combination of Syto 9 (SYTO™ 9 Green Fluorescent Nucleic Acid Stain) and propidium iodide was confirmed to be effective for the determination of viability of biofilm-forming microbial cells. The combination of...
Rotavirus as an agent of diarrheal diseases
Hampl, Tomáš ; Vejsová, Marcela (advisor) ; Konečná, Klára (referee)
Author: Tomáš Hampl Title: Rotavirus as an agent of diarrheal diseases Bachelor thesis Charles University in Prague, Faculty of Pharmacy, Hradec Králové Field of study: Medical Laboratory Background: This bachelor thesis aims at dealing with rotavirus issues, taxonomically classifying them, describing their structure, properties, origin and course of the diseases they cause. In the experimental part the thesis will deal with the epidemiology of rotavirus infections at the Faculty Hospital in Hradec Králové in the period between 2010-2017 and their evaluation. Main findings: Rotaviruses are very often the cause of serious and dangerous diarrhea in children up to 5 years of age, including infants and toddlers. According to the WHO, more than half a million children worldwide die each year, where children will get ill around 150 million. Therefore, early diagnosis and appropriate therapy are very important. An adult can also become infected, the course of the disease is much milder than children. Conclusions: Important prevention of the origin and spread of rotavirus infections requires careful personal hygiene, especially hand washing. Because infections are the most common in children's groups, disinfection of the environment, toys, furniture and other objects is necessary in case of spread of...
Vaginal microbiota and its effect in pregnancy
Mašátová, Eva ; Vejsová, Marcela (advisor) ; Konečná, Klára (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Eva Mašátová Trainer: Mgr. Marcela Vejsová, Ph.D. Title: Vaginal microbiota and its effect in pregnancy This work was created to summarize the knowledge of the vaginal microbiota in general and also during the pregnancy. The work deals with the description of individual microorganisms naturally occurring in the vaginal environment and their occurrence in the population. Diagnostic methods and techniques used in gynecology for examination of the vaginal environment are also described. The last part describes the antimicrobial treatment and its changes during the pregnancy. The vaginal microbiota is a diverse ecosystem of every woman that changes during her lifetime with a variety of influences. During the pregnancy, the risk of serious complications, such as premature birth or infections, is significantly increased when the patient is colonized. If the patient is colonized, it is not possible to definitely determine whether it is a pathological condition or not. Representatives of the genus Streptococcus, Staphylococcus, Gardnerella, Candida, Ureaplasma, Mycoplasma and others are naturally present in the vaginal environment. The pathogen is securely proven mainly by the density of...
In vitro screening of novel potentially active antibacterial compounds II
Zbořilová, Eliška ; Janďourek, Ondřej (advisor) ; Vejsová, Marcela (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Bc. Eliška Zbořilová Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of novel potentially active antibacterial compounds II Key words: antibiotics, bacteria, resistance, minimal inhibitory concentration, bacterial susceptibility to antibiotics This diploma thesis deals with testing of the efficacy of new antimicrobial agents on selected representative bacteria. The work consists of a theoretical part and a practical part. The theoretical part includes the history and the present of antibiotics describing current issues and the layout of the antibiotic policy in the Czech Republic and in the world. It deals with the characteristics of drugs, below which are described their importance, division, effects, resistance. Furthermore, this part of work describes detailed mechanism of action for two selected groups of compounds. Last but not least, this work portrays the methods that are used to determine the sensitivity of bacteria to antibiotics. The practical part deals with the description of the whole work process, including the characteristics of used bacterial strains and the list of tested substances....
Meningococcal infection
Malá, Markéta ; Vejsová, Marcela (advisor) ; Konečná, Klára (referee)
7 2. ABSTRACT Title of Bachelor thesis: Meningococcal infections Student: Markéta Malá Supervisor: Mgr. Marcela Vejsová, Ph.D. Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Backround: The aim of this bachelor thesis was to summarize the findings about meningococcal infections and their originator - Neisseria meningitidis. Further this bachelor thesis should provide infomation about progression in meningococcal vaccines development, treatment and diagnostics. Main findings: N. meningitidis spreads into the human body from nasopharynx, through the epithelium it travels into the bloodstream. Severity of meningococcal infection depends on a strain of meningococcus and on strenght of immune system on the side of a host. Meningococcus has got efective tools for invading human body, they are called virulence factors. Quick recognition of clinical signs and rapid confirmation of infection originator are important when the infection breaks out. Nowadays we can prevent meningococcal infection by effective vaccination - combining meningococcal tetravalent conjugate vaccine and meningococcal serogroup B vaccine. Conclusions: Infections caused by N. meningitidis varies from ordinary nasopharyngitis to bacteremia and severe acute meningitis. N. meningitidis...

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