National Repository of Grey Literature 47 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Characteristic of chromosomal changes in nephroblastomas using SNP array and MLPA
Štolová, Lucie ; Vícha, Aleš (advisor) ; Daňková, Pavlína (referee)
Nephroblastoma is the most prevalent pediatric kidney tumor, which occurs primarily in younger children with the average age at diagnosis of 42,5 months for girls and 36,5 months for boys. Even though its treatment is currently very succesful and the overall survival rate reaches over 90 %, there are still more things to be discovered and improved. An important role for the right choice of treatment plays not only the histology of tumor, but also the chromosomal changes present at tumor. Some of them (for example 1q gain, simultaneous deletion of 1p and 16q, TP53 deletion) were confirmed as negative prognostic markers because they are associated with an increased risk of relapse or with anaplastic type of nephroblastoma that is included in a high risk group. These changes are therefore used together with the tumor histology for stratification of nephroblastomas. Some of these changes were found in a heterogeneous state (only in a part of the cells) in nephroblastoma, which also complicates the treatment of the patient and which cannot be solved when only one sample is taken from the tumor. In this work we concentrated on the detection of chromosomal changes present in nephroblastomas of 44 patients and their associations with clinical data. We have proved some of the known associations (22q...
Effect of selected inflammatory agents on the osteoclastogenesis
Škubica, Patrik ; Daňková, Pavlína (advisor) ; Hušáková, Markéta (referee)
Introduction: Bone is a highly active tissue throughout life and is a subject to constant remodelling. Main cells responsible for continuous resorption and de novo synthesis of bone matrix are osteoclast, osteoblasts and osteocytes. Osteoclasts are the only known type of cells able to resorb bone. These cells are formed by fusion of precursor cells in bone marrow or peripheral blood in a process called osteoclastogenesis. Formation of osteoclasts may be of importance concerning chronic inflammatory diseases that are linked with higher risk of developing osteoporosis during lifespan. Celiac disease is one of those diseases, which is characterized by destruction of intestinal mucosa after ingestion of gluten by susceptible individuals followed by induction of chronic inflammation. In this work, we focused on the potential role of osteoclastogenesis in the development of osteoporosis in patients with celiac disease and we studied roles of selected inflammatory agents (TNF-α, IL-6, IFN-γ a cfDNA) with supposed or hypothesised effects on osteoclastogenesis. Material & Methods: We obtained plasma and serum samples from newly diagnosed patients with celiac disease, patients on gluten free diet and healthy controls and analysed concentrations of cfDNA and inflammatory cytokines TNF-α, IL-6 and IFN-γ in...
Determination of inflammatory markers of the eye based on the analysis of tears - potential and limits
Mandíková, Šárka ; Daňková, Pavlína (advisor) ; Sičáková, Silvia (referee)
In this study, we aimed to determine the levels of cytokines IL-1β, IL-4, IL-10, IFN-γ, MIF and VEGF in tears derived from healthy subjects. We tested cytokines as potential markers of inflammation for their potential use in clinical practice. Having reliable method for measuring cytokine levels in tears would enable an early diagnosis of eye diseases. In two phases, cytokines in tears of healthy individuals were analyzed using Bio-Plex Cytokine Assay (Bio-Rad). We assessed the suitability of methods for diagnostic purposes as well as the suitability of our selected cytokines. Statistically significant positive correlations of cytokines were confirmed: IL-10 with IFN-γ (r = 0,81), MIF with VEGF (r = 0,42 / r = 0,49), IL-1β with IL-10 (r = 0,52), IL-1β with IFN-γ (r = 0,55), IL-1β with VEGF (r = 0,38), IFN-γ with VEGF (r = 0,45) and IL-4 with VEGF (r = 0,48) in healthy subjects in tears. IL-4 (r = -0,37) and IFN-γ (r = -0,42) correlate negatively with age. In healthy individuals, there seem to be no differences with regard to gender, BMI, body fat, time of meal consumption prior to tear collection, eye strain when using a computer, dry eyes. Thus, studied cytokines are suitable for diagnostic purposes. Significant differences in concentrations of four (IL-1β, IL-10, IFN-γ a VEGF) of the five...
Celiac disease-related inflammatory factors and their effect on peripheral blood monocytes
Golovina, Elena ; Daňková, Pavlína (advisor) ; Čepek, Pavel (referee)
Introduction: Celiac disease is an organ-specific autoimmune disease that results in loss of oral tolerance of gluten in genetically predisposed individuals. Increased levels of inflammatory cytokines (eg IFN-γ, TNF-α, IL-6) in the small intestine and peripheral blood have been observed in patients with celiac disease. These cytokines have the ability to increase the expression of interferon regulatory factor 1 (IRF1), which is a potential player in the pathogenesis of celiac disease. IRF1 plays a role in apoptosis, differentiation and anti- inflammatory response of various immune cells. Monocytes are an important component of the immune system. A wide variety of their functions allow them to exertmembrane receptors, whose expression may cause, among other things, by IRF1. To clarify the effects of IRF1 on the pathogenesis of celiac disease we have focused on circulating monocytes in this pilot study and (i) detection changes in the monocytic expression of IRF1 mRNA in inflammatory environments in patients with celiac disease (recent, rCD and adherent one year and longer gluten-free diet, CD-GFD), and ii) subsequently to reveal the effect of IRF1 on monocytes by apoptosis markers (CD95) and anti-inflammatory responses markers (CD163 and IL-10). Material and methods: The study includes 15 patients...
Expression profiles of selected celiac disease candidate genes
Okruhlicová, Šárka ; Daňková, Pavlína (advisor) ; Novota, Peter (referee)
Introduction: Celiac disease is a multifactorial autoimmune disease that is caused by a response to gluten and related proteins in genetically susceptible individuals. Genetic studies have demonstrated a high association of celiac disease with HLA genes (human leukocyte antigens) II. Class. Approximately 90-95% of the patients have a HLA-DQ2 haplotype (DQA1*05/DQB1*02), the remaining 5-10% are carriers of HLA-DQ8 (DQA1*03/DQB1*03) or inherited only one allele of HLA-DQ2 genotype. Approximately 30% of the healthy population has this haplotype and only 1% develops celiac disease. If 30% of healthy individuals have this haplotype and do not develop celiac disease, this may be affected by a different level of expression in healthy and diseased individuals. Aims: The aim was to design and test a primer and probe system for QPCR that will amplify all selected HLA-DQA1 and HLA-DQB1 candidate genes at risk for celiac disease and use this system to measure the relative level of expression of risk genes in healthy donors and patients with celiac disease. Methods: The study included 10 patients with recent celiac disease and 15 healthy donors who were selected from the database of the Institute of Medical Genetics of the 3.LF UK based on their genotypes HLA-DQA1 and HLA-DQB1. Patients were first detected...
Genetic determination and heredity of craniofacial traits based on specific DNA loci
Králíková, Kristýna ; Daňková, Pavlína (advisor) ; Šimková, Halina (referee)
Introduction: Genetic determination of human face is clearly visible in family members. The resemblance between monozygotic twins who are genetically identical is especially remarkable. So far the possibilities of reliable prediction of the complex morphology of facial traits on the basis of genome analysis and the ability to capture the variability of human facial morphology through genotype variability are highly limited. Complete genetic basis of the physiological variability of craniofacial traits remains more or less unknown. This master's thesis was created as a pilot study of the shared project of the Laboratory of 3D Imagining and Analytical Methods and the Laboratory of Molecular Anthropology on Department of Anthropology and Human Genetics. Material and Methods: The specimen collection is composed of DNA samples derived from 30 families (29 with 4 members, 1 with 5 members) who fulfilled required criteria. Nine single nucleotide polymorphisms were chosen based on the available information. Eight of them are linked to normal facial variability and one was chosen based on the assumed function of the gene where the polymorphism is located. There were two methods of genotyping: RFLP method with the use of restriction endonuclease and SNaPshot method. Morphological data were provided by the...
Expression profiles of selected celiac disease candidate genes
Okruhlicová, Šárka ; Daňková, Pavlína (advisor) ; Novota, Peter (referee)
Introduction: Celiac disease is a multifactorial autoimmune disease that is caused by a response to gluten and related proteins in genetically susceptible individuals. Genetic studies have demonstrated a high association of celiac disease with HLA genes (human leukocyte antigens) II. Class. Approximately 90-95% of the patients have a HLA-DQ2 genotype (DQA1 * 05 / DQB1 * 02), the remaining 5-10% are carriers of HLA-DQ8 (DQA1 * 03 / DQB1 * 03) or inherited only one allele of HLA-DQ2 genotype. Approximately 30% of the healthy population has this genotype and only 1% develops celiac disease. If 30% of healthy individuals have this genotype and do not develop celiac disease, this may be affected by a different level of expression in healthy and diseased individuals. Aims: The aim was to design and test a primer and probe system for QPCR that will amplify all selected HLA-DQA1 and HLA-DQB1 candidate genes at risk for celiac disease and use this system to measure the relative level of expression of risk genes in healthy donors and patients with celiac disease. Methods: The study included 10 patients with recent celiac disease and 15 healthy donors who were selected from the database of the Institute of Medical Genetics of the 3.LF UK based on their genotypes HLA-DQA1 and HLA-DQB1. Patients were first...
The use of molecular-biology methods (QRT-PCR) and immunocytological methods (flow cytometry and immunocytochemistry) for the detection of minimal residual disease in neuroblastoma
Grüncveigová, Veronika ; Vícha, Aleš (advisor) ; Daňková, Pavlína (referee)
With a continuous development of molecular-biology methods more attention has been paid to molecular detection of minimal residual diseases in solid tumors. In our study we focused on detection of MRD in neuroblastoma. Neuroblastoma is one of the peripheral neuroblastic tumors (pNTs) that accounts approximately for10 percent of all childhood cancers. The question raised however not answered until this day is whether evidence of MRD in bone marrow may be used as independent prognostic factor in diagnosis of neuroblastoma. Furthermore, it is important to establish what kind of testing technique should be used and what values to look at. There exist various methodologies in detection of MRD evidence in neuroblastoma. These methods differ in cost and complexity, but mainly some of them are more specific and sensitive than the other. Cancer cells may be detected in the blood as well as in the bone marrow. Very often it is the bone marrow that is affected by the metastasis in neuroblastoma, therefore 85% of all high risk neuroblastomas show positive results in the standard cytomorphology tests of bone marrow. Low numbers of cancer cells in bone marrow or peripheral blood (especially during or after the end of treatment) are below the standard values of detection limit in most of the classic methodologies...

National Repository of Grey Literature : 47 records found   1 - 10nextend  jump to record:
See also: similar author names
1 DANKOVÁ, Pavla
2 DAŇKOVÁ, Patricie
4 DAŇKOVÁ, Petra
4 Daňková, Petra
Interested in being notified about new results for this query?
Subscribe to the RSS feed.