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Modulation of mesenchymal stem cell properties and their use in the regulation of transplantation immunity
Peřinová, Lucie ; Krulová, Magdaléna (advisor) ; Zajícová, Alena (referee)
Mesenchymal stem cells (MSCs) represent a heterogeneous population of stromal cells with a pluripotent differentiation potential. They can be isolated from multiple tissues of mesodermal origin, such as bone marrow, adipose tissue, umbilical cord blood and peripheral blood and afterwards externally expanded according their adherence to the plastic surfaces. These cells show remarkable immunomodulatory properties, suppressing T-, B- and NK-cell functions, and also modulating dendritic cell activities and influencing immune responses during tissue repair and recovery. MSCs have been shown to possess ability to migrate to sites of inflammation and tissue injury. All these properties make MSCs a promising tool for clinical application. Our primary goal was to identify processes that may influence immunoregulatory effects of MSCs. In order to promote immunossupressive qualities of MSCs we established the scheme comprising MSCs precultivated with various cytokines and Toll-like receptors (TLR) ligands in vitro, with the final aim to improve the therapeutic effect of MSCs on wound healing in vivo. We studied modulation of MSCs properties and consequently the effect of influenced MSCs on cells of the immune system. The immunosuppression is mainly mediated through secreted factors that MSCs produced after...
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Enhancement of the efficacy of DNA vaccines against the cervical cancer with helper epitopes
Peřinová, Lucie ; Brábek, Jan (referee) ; Šmahel, Michal (advisor)
The human papillomaviruses (HPV) are the etiological agent of cervical cancer. Their oncoproteins E6 and E7 are involved in the transformation of an infected cell into a neoplastic cell, thereby they are the target antigenes for the development of DNA vaccines. Helper epitopes activating CD4+ T cells are under study because they enhance the efficacy of DNA vaccines through increasing the number of cytotoxic T lymphocytes and thereby removal of the tumor. There are already being used epitopes derived directly from oncoproteins, synthetic epitopes or bacterial epitopes for the general enhancement of the immune response. Sufficient number of comparative studies which would establish the exactly most efficient helper epitopes has not been made. The research aims at combining more peptide types using immunostimulatory molecules.
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Cycling the Camino de Santiago
Perinová, Lucie ; Netková, Jarmila (advisor) ; Bakos, Tomáš (referee)
The aim of this paper is to present the St. James Ways as an important product of modern tourism and focus on the pilgrim routes, specifically on the route Vía de la Plata, which will be analyzed from the cyclist point of view. The first part of this paper deals with the definition of the pilgrimage tourism, describes some of the most interesting pilgrim places, presents the history of the pilgrimage. The following chapter presents the main statistic data about tourism in Spain, information about the St. James Ways, including their history and development and the forms of promotion in general. The next part explains the historic and religion background, presents the practical information, and forms of promotion of the Vía de la Plata, simultaneously analyzes the focus on the cyclist as a specific tourist segment. The last part deals with the official pilgrimage statistic data and confirms the increasing interest in the St. James Ways. Substantial are the results of the survey, representing how the cycling segment is interested in this form of modern tourism.
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