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Deactivation of the Intramolecular Charge Transfer state in RC-LH1 complexes of genetically modified purple bacteria \kur{Rhodobacter sphaeroides}
ŠÍMOVÁ, Ivana
The main task of this thesis is to find the ICT activator of a carotenoid spheroidenone in the pigment-protein complex LH1 of Rhodobacter sphaeroides. The ICT state is characterized by a red-shifted positive transient absorption signal around 750 - 800 nm. We hypothesize that the presence of the ICT state is associated with the pigment-protein interaction of a carotenoid keto group and specific amino acid in the protein. To perform the experiment, four mutant complexes with amino acids substitutions, which should result in decreased intensity of the ICT signal due to a weaker or missing pigment-protein interaction, were obtained and measured using femtosecond time resolved pump-probe spectroscopy.
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Domestic violence as a social problem in České Budějovice district
ŠÍMOVÁ, Ivana
Violence at home, a.k.a. home violence, is a spiraling cycle of brutality committed in private between closely related persons. Such violence can by physical, psychological, sexual, economic or social, with the roles of the attacker and the victim clearly defined and never changing. Anyone can be exposed to home violence, irrespective of her or his social position, education, job, sex, age, religion, or the type of partnership that people live in. Advanced societies have developed a tendency to tackle the issue, and the awareness of the problem has improved. In our country this favorable development is driven primarily by the newly enacted regulations and the keener interest stirred up in the public. The thesis aimed to investigate the general public awareness of home violence within the District of České Budějovice. As followed from the survey, the public living in the District is well aware of the issue. The results showed that as much as 69% of respondents knew about home violence, while the remaining 31% were ignorant of the problem. Hypothesis: Females are better informed about home violence than males - confirmed by comparison between what the women and the men knew about the issue. The difference encountered, however, was just minor. Hypothesis: People living in the District of České Budějovice are not knowledgeable about changes newly passed in Act 135/200 to solve the problem of violence at home - not confirmed. The majority of respondents heard of the Act and were able to specify at least the essential changes it enacted. The change the respondents mentioned most often was the 10 day period of flat-eviction order. It comes as a matter of interest that males were better aware of the Act and could better quote the changes it introduced. Hypothesis: The public accepts as true that home violence is confined to just problem-ridden families - not confirmed. Eighty seven percent of females and 81% of males were convinced that home violence was not limited to only such families. Merely 13% of females and 19% of males believed the home violence was a matter of the problem-ridden families. The survey results showed that the public living in the District of České Budějovice had dropped this myth, i.e. they had not seen the hypothesis as truthful. The thesis can serve as a source of information for the lay public, as a piece of instruction material for the students of the University of South Bohemia, or it could also be given to the Intervention Center to be distributed among the victims of home violence.
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