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Examination of the Spatial Structure of Pigs' Melanoma in Tissue Sections Based on Histology and Mass Spectrometry
Anýz, J. ; Vysloužilová, L. ; Horák, Vratislav ; Štěpánková, O. ; Vaculovič, T. ; Adam, V.
We examine the spatial structure of the melanoma in tissue sections. The pigs melanoma was examined in 10 tissue samples obtained from animals of age between 4 and 22 weeks. The tissue sections were measured by light microscopy and Laser Ablation Ion Inductively Coupled Plasma Mass Spectrometry to obtain spatial metal (Cu, Zn) distribution. The exploratory analysis of the tissue sections indicates there is clearly a pattern in the spatial structure. Different projections of the spatial structure of the melanoma are obtained by the different measurement methods. The spectral clustering on the data was utilized to describe the structure in the data. According to the clustering results, there are distinct clusters of observations in the histological data. The spatial elemental distribution of the metals Cu and Zn cannot be clustered -the data form one compact cluster. The clustering of the histological images produces clusters which are related to the annotation of the biological samples in broader terms -the differences between fibrous and cancerous tissue.
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Phenotypic Analyses of the HD Transgenic Minipig Model (A11609)
Ellederová, Zdeňka
The transgenic Huntington's disease minipigs (TgHD) express N‐terminal part of human mutated huntingtin (124Q) under the control of human huntingtin promoter. The founder animal, born in 2009, gave birth to four subsequent generations with an equal contribution of wild‐type (WT) and transgenic (TgHD) piglets in all litters. The model is being used for preclinical huntingtin lowering studies. Here we take different non-invasive and invasive approaches, some of which are unique for large animal models, to study the phenotype development comparing WT and TgHD siblings. We show gradual progression of the disease in these TgHD animals. Moreover, some biomarkers were identified. These markers could serve for monitoring of organism response to HD treatment to assess efficacy and safety in preclinical studies prior to human clinical trials.
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Bacteriophage 936 by qPCR quantification in dairy products.
Olša Fliegerová, Kateřina ; Mrázek, Jakub ; Štrosová, Lenka ; Němečková, I. ; Kopečný, Jan
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod of phage monitoring and quantification necessary part of phage prevention. Method is based on qPCR amplification and fluorescent detection of copies number of the phage main\nstructural protein and therefore is independent on the phage cultivation. It is quick, reliable a sensitive enough to estimate phage copy numbers in dairy starters and whey in any phase of dairy production. \n
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Results of nanomaterials testing for skin wounds nursing
Juhás, Štefan ; Juhásová, Jana
Using minipigs experiments, we tested the healing of fresh wounds treated with different nanofibrous materials and compared them with standard treatment. From predefined wounds, samples were taken at regular intervals for histological analysis, microbiology, wound measurements and photodocumentation. Blood was also collected from the miniprasate to determine pro-inflammatory cytokines.
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