Original title: Thin Nanostructured Film with Bioapplication Potential
Authors: KUMAR, Sanjay
Document type: Doctoral theses
Year: 2024
Language: eng
Abstract: The Ph.D. thesis aims to investigate, develop, and fabricate advanced nanostructured surfaces for (bio)sensors and detection applications. The primary outcome of the research is the development of a functional sensor transducer for the detection of specific anti-DbpA antibodies, typically produced by a living organism in response to Lyme disease infection, as well as for detecting live Borreliosis pathogens. The developed sensor is based on AgNPs/C:H:N:O plasma polymer nanocomposites. Silver (Ag) nanoparticles, generated by a gas aggregation source, are incorporated into a C:H:N:O plasma polymer matrix, which is deposited by magnetron sputtering of nylon 6.6. The surface of the AgNPs/C:H:N:O nanocomposite is functionalized with amino-rich -NH2 grafts and specific antibodies to achieve high specificity in detection. Anchored Ag nanoparticles propagate localized surface plasmon resonance (LSPR), optically responding to changes caused by immobilized targets near the nanoparticles. The sensitivity of the sensor transducer is enhanced by incorporating Cu nanoparticles, which contribute to Cu-Ag coupling modes responsible for the modulation of the LSPR bands. The achieved results pave the way for rapid, low-cost, sensitive platforms for detecting not only the antibodies/pathogens studied here but also potentially other targets.
Keywords: adhesion; annealing; antibacterial; Borrelia; C:H:N:O thin film; Cu); enhanced detection; immobilization; localized surface plasmon resonance (LSPR); Lyme disease; magnetron sputtering; nanocomposite; nanoparticles (Ag; nanostructures; nylon; plasma polymer; plasma polymerization; plasma-assisted deposition; plasmonic absorption; sensors; surface functionalization
Citation: KUMAR, Sanjay. Thin Nanostructured Film with Bioapplication Potential. České Budějovice, 2024. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/59343

Permalink: http://www.nusl.cz/ntk/nusl-651523


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Doctoral theses