Název: Kininase-like metalloprotease regulates tick salivation
Autoři: FOITLOVÁ, Denisa
Typ dokumentu: Diplomové práce
Rok: 2024
Jazyk: eng
Abstrakt: Ticks are obligate hematophagous ectoparasites that require a blood meal to complete their development and reproduction. To facilitate blood feeding, they secrete saliva, a complex mixture of bioactive compounds that modulate the host's immune responses. To evade host immunity, ticks have evolved their salivary proteins to exhibit low immunogenicity. However, in some less adapted hosts, compounds of tick saliva can evoke antibody-mediated responses. Angiotensin-converting enzyme-like kininase (Ir-ACE), the main subject of this thesis, was identified as one of the most dominant salivary antigens of the tick Ixodes ricinus. The aim of this thesis was to investigate the molecular and functional properties of this enzyme; an objective that has not yet been accomplished. To achieve this, RNAi and repeated feeding experiments, proteomic analysis of salivary glands and expression of recombinant Ir-ACE used for the vaccination experiments and substrate profiling were performed.
Klíčová slova: angiotensin-converting enzyme-like kininase; metalloproteases; saliva; salivary glands; ticks
Citace: FOITLOVÁ, Denisa. Kininase-like metalloprotease regulates tick salivation. České Budějovice, 2024. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Instituce: Jihočeská univerzita v Českých Budějovicích (web)
Informace o dostupnosti dokumentu: Plný text je dostupný v digitálním repozitáři JČU.
Původní záznam: http://www.jcu.cz/vskp/67667

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-623884


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Vysokoškolské kvalifikační práce > Diplomové práce