Original title: Využití kapalinové chromatografie ve farmaceutické analýze II
Translated title: Use of liquid chromatography in pharmaceutical analysis II.
Authors: Sovič, Samuel ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Document type: Master’s theses
Year: 2023
Language: slo
Abstract: [eng] [cze]
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Samuel Sovič Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: The use of liquid chromatography in pharmaceutical analysis II The diploma thesis deals with the analysis of the retention behavior of selected antiepileptics: Gabapentin, Levetiracetam, Primidone and Lamotrigine. Three different C18 stationary phases were tested such as Phenyl; PFP; Kinetex, different mobile phase composition, column temperature and suitable wavelengths in the range of 210-220 nm for all analytes. The optimal analysis conditions were on an Agilent Eclipse XDB-Phenyl 5 μm column (4.6 mm x 150 mm), at a temperature of 40 řC and a flow rate of MP at 0.8 ml/min, at a wavelength of 220 nm. By creating a gradient, the analysis was shortened from 15 minutes with isocratic elution to 9 minutes. The method was partially validated in terms of linearity, accuracy, precision, selectivity and robustness. After that, the method was applied for real samples of patients from FN HK. Univerzita Karlova, Farmaceutická fakulta v Hradci Králové Katedra: Katedra farmaceutické chemie a farmaceutické analýzy Kandidát: Samuel Sovič Školiteľ: PharmDr. Pavla Pilařová, Ph.D. Názov diplomovej práce: Využitie kvapalinovej chromatografie vo farmaceutickej analýze II Diplomová práca sa zaoberá analýzou retenčného chovania vybraných antiepileptík: Gabapentin, Levetiracetam, Primidon a Lamotrigin. Boli testované tri rôzne C18 stacionárne fázy Phenyl ; PFP; Kinetex, rôzne zloženie mobilnej fázy, teplota na kolóne a vhodné vlnové dĺžky v rozmedzí 210-220 nm pre všetky analyty. Optimálne podmienky analýzy boli na kolóne Agilent Eclipse XDB-Phenyl 5 μm (4,6mm x 150mm), pri teplote 40 řC a prietoku MF 0,8 ml/min, pri vlnovej dĺžke 220 nm. Vytvorením gradientu bola analýza skrátená z 15 minút pri izokratickej elúcii na 9 minút. Metóda bola čiastočne validovaná z hľadiska linearity, presnosti, precíznosti, selektivity a robustnosti. Ďalej bola metóda aplikovaná na reálne vzorky pacientov z FN HK.

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/185581

Permalink: http://www.nusl.cz/ntk/nusl-535458


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses