Original title: An Enzymatic Biosensor with Amperometric Detection in a Flow Injection Analysis for the Determination of L-lactic Acid: Development and Application
Authors: Tvorynska, Sofiia ; Barek, J. ; Josypčuk, Bohdan
Document type: Papers
Conference/Event: Moderní elektrochemické metody /42./, Jetřichovice (CZ), 20230522
Year: 2023
Language: eng
Abstract: An amperometric biosensor consisting of an enzymatic mini-reactor (lactate oxidase covalently\nattached to −NH2 functionalized mesoporous silica powder SBA−15 using glutaraldehyde) and\na silver amalgam-based screen-printed electrode acting as a transducer was developed for the\ndetermination of L-lactic acid (LA) in FIA. The detection potential of −0.9 V vs. Ag pseudoreference\nelectrode was applied for cathodic detection of enzymatically consumed oxygen.\nUnder the optimized conditions, the constructed biosensor enabled selective determination of\nLA with a micromolar limit of detection. Importantly, the proposed biosensor represented\nexcellent operational stability after ≥350 measurements. Finally, it was successfully applied to\nreal sample analysis.
Keywords: Amperometric biosensor; L-lactic acid; Lactate oxidase; silver amalgam
Project no.: GA20-07350S (CEP)
Funding provider: GA ČR
Host item entry: Moderní elektrochemické metody /42./. Sborník přednášek, ISBN 978-80-908947-0-9

Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: https://hdl.handle.net/11104/0343145

Permalink: http://www.nusl.cz/ntk/nusl-525318


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Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers