Original title: Design and production of TBEV specific cleavage reporter for in vitro and in cell studies
Authors: KOTOUNOVÁ, Eliška
Document type: Master’s theses
Year: 2021
Language: eng
Abstract: Tick-borne encephalitis virus (TBEV) is an important human pathogen and as of now, there are no specific therapeutics to combat this disease. In vitro and in cell models are essential in studying the disease and in the pursuit of novel therapeutics. This thesis deals with the design and production of two genetically encoded cleavage reporters of TBEV NS2B-NS3 protease activity. The reporters were produced in E. coli and tested with recombinant NS2B-NS3 protease, as well as produced in mammalian cell lines and analyzed for fluorescence upon infection with TBEV.
Keywords: cleavage reporter; NS2B-NS3 protease; PAmCherry; TBEV
Citation: KOTOUNOVÁ, Eliška. Design and production of TBEV specific cleavage reporter for in vitro and in cell studies. České Budějovice, 2021. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/63434

Permalink: http://www.nusl.cz/ntk/nusl-517757


The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses