Original title:
Primer Extension Reaction at the Gold Electrode Surface
Authors:
Vidláková, Pavlína ; Havran, Luděk ; Fojta, Miroslav Document type: Papers Conference/Event: Moderní elektrochemické metody /35./, Jetřichovice (CZ), 20150518
Year:
2015
Language:
eng Abstract:
One option of covalent DNA labeling is incorporation of chemically modified nucleotides using corresponding deoxynucleotide triphosphates into DNA molecules using polymerase-catalyzed primer extension. Primer extension is usually performed in solution and then the product is analyzed, often after a suitable separation. In our study we tested the possibility to prepare oligonucleotides with enzymatically incorporated anthraquinone labeled nucleotides using primer extension on templates immobilize at a gold electrode surface.
Keywords:
dna; immobilization; optimization Project no.: GAP206/11/1638 (CEP) Funding provider: GA ČR Host item entry: XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference, ISBN 978-80-905221-3-8
Institution: Institute of Biophysics AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0297907