Original title: Primer Extension Reaction at the Gold Electrode Surface
Authors: Vidláková, Pavlína ; Havran, Luděk ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /35./, Jetřichovice (CZ), 20150518
Year: 2015
Language: eng
Abstract: One option of covalent DNA labeling is incorporation of chemically modified nucleotides using corresponding deoxynucleotide triphosphates into DNA molecules using polymerase-catalyzed primer extension. Primer extension is usually performed in solution and then the product is analyzed, often after a suitable separation. In our study we tested the possibility to prepare oligonucleotides with enzymatically incorporated anthraquinone labeled nucleotides using primer extension on templates immobilize at a gold electrode surface.
Keywords: dna; immobilization; optimization
Project no.: GAP206/11/1638 (CEP)
Funding provider: GA ČR
Host item entry: XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference, ISBN 978-80-905221-3-8

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297907

Permalink: http://www.nusl.cz/ntk/nusl-396428


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers