Original title: Improved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation application
Authors: DVOŘÁKOVÁ, Hana
Document type: Rigorous theses
Year: 2019
Language: eng
Abstract: Spray application of 5azacytidine on established plant seedlings was tested for its demethylating efficiency. It represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethylating agent through germination of seeds in its solution. Further, the 5azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.
Keywords: 5-azacytidine; competition; demethylation; DNA methylation; ecological epigenetics; ecology; plant memory; plant performance; toxicity; transgenerational effects
Citation: DVOŘÁKOVÁ, Hana. Improved demethylation in ecological epigenetic experiments: Testing a simple and harmless foliar demethylation application. České Budějovice, 2019. rigorózní práce (RNDr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/56630

Permalink: http://www.nusl.cz/ntk/nusl-391805


The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Rigorous theses