Original title: Uspořádání mikrotubulárního cytoskeletu během zakládání Casparyho proužku v endodermálních buňkách kořene Allium cepa
Translated title: Organization of the microtubular cytoskeleton during the formation of Casparian band in root endodermal cells of Allium cepa
Authors: Macháčová, Kateřina ; Soukup, Aleš (advisor) ; Lux, Alexander (referee)
Document type: Master’s theses
Year: 2008
Language: cze
Abstract: 103 7 Summary Organization of the Microtubule Cytoskeleton during the Formation of a Casparian Band in Root Endodermal Cells of Allium cepa This thesis is focused on the process of Casparian bands initiation in radial and transverse cell walls of the root endodermis of Allium cepa. The Casparian band is characterized by a chemical modification of the cell wall in the position of the Casparian band followed by the formation of the tight binding of a plasma membrane to the cell wall. We assume that cortical microtubules can play an important role in both processes searching for changes of cortical array during the development of proendodermis and during the foundation of the Casparian band in the endodermis. The classical method of the microtubules visualisation in roots was modified by the application of microwaves during the process of fixation. The tissues were observed with the fluorescence and confocal microscopy. Image postprocessing and construction of 3D-models were subsequently used. Proendodermal cells in Allium cepa are recognized for the first time among other root cells in the distance of about 2 mm from the root tip. The Casparian band is firstly detected in the distance of about 8 mm from the root apex by means of autofluorescence of the Casparian band. The cell wall of the Casparian band...

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/104173

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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses