Original title: Fluorescence kinetic microscopy: 2D imaging of variable chlorophyll fluorescence from single algal cells with spectral resolution
Translated title: Fluorescenční kinetická mikroskopie: 2D zobrazování variabilní fluorescence chlorofylu jednotlivých buněk řas se spektrálním rozlišením
Authors: Prášil, Ondřej ; Šetlíková, Eva ; Veselka, D.
Document type: Papers
Conference/Event: Aquafluo - Chlorophyll Fluorescence in Aquatic Sciences Meeting, Nové Hrady (CZ), 2007-05-28 / 2007-06-01
Year: 2007
Language: eng
Abstract: [eng] [cze]
The fluorescence kinetic microscopy (FKM) provides new dimension in application of chlorophyll fluorescence techniques. It combines classical fluorescence microscopy with 2D kinetic detection systems. FKM allows to examine photosynthesis of individual algal cells or on the cellular level of macroscopic structures. FKM is well suited to study heterogeneity in photosynthetic response among individual cells in algal cultures or in natural mixed populations. Special microscopic chambers allow to maintain live cells under optimal conditions for several hours or days and to follow evolution of photosynthesis in individual cells during the course of their growth cycle. Recent technical developments have allowed to determine FKM fluorescence kinetic with spectral resolution Předmětem workshopu je seznámení se s technikou fluorescenční kinetické spektroskopie s 2D rozlišením, pro charakterizaci fotosyntetické aktivity jednotlivých buněk řas a sinic
Keywords: fluorescence; kinetic; microscopy
Project no.: CEZ:AV0Z50200510 (CEP), 1P05ME824 (CEP)
Funding provider: GA MŠk
Host item entry: Aquafluo - Chlorophyll Fluorescence in Aquatic Sciences Meeting

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0159795

Permalink: http://www.nusl.cz/ntk/nusl-38639


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Research > Institutes ASCR > Institute of Microbiology
Conference materials > Papers